Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage

Knut Rudi, Irina Hagen, Bente Carina Johnsrud, Guro Skjefstad and Ingun Tryland
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Knut Rudi: Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway
Irina Hagen: Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway
Bente Carina Johnsrud: Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway
Guro Skjefstad: Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway
Ingun Tryland: NIVA Norwegian Water Research Institute, Oslo, Norway

IJERPH, 2010, vol. 7, issue 9, 1-6

Abstract: We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing.

Keywords: viable/dead cells; UV; quantitative PCR (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2010
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