Quantitative Variations of Intracellular Microcystin-LR, -RR and -YR in Samples Collected from Four Locations in Hartbeespoort Dam in North West Province (South Africa) During the 2010/2011 Summer Season

Elbert A. Mbukwa, Titus A.M. Msagati and Bhekie B. Mamba
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Elbert A. Mbukwa: Department of Applied Chemistry, Faculty of Science, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, Johannesburg, Republic of South Africa
Titus A.M. Msagati: Department of Applied Chemistry, Faculty of Science, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, Johannesburg, Republic of South Africa
Bhekie B. Mamba: Department of Applied Chemistry, Faculty of Science, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, Johannesburg, Republic of South Africa

IJERPH, 2012, vol. 9, issue 10, 1-22

Abstract: The Hartbeespoort (HBP) Dam is a reservoir used for agricultural, domestic supply of raw potable water and recreational activities in South Africa’s North-West Province. Eutrophication and cyanobacterial blooms have long been a cause of water-quality problems in this reservoir. The most prevalent bloom-forming species is Microcystis aeruginosa , often producing the toxin microcystin, a hepatotoxin which can negatively impact aquatic animal and human health, and poses a problem for potable water supply. Algal samples were collected monthly from four pre-determined sites in the dam during the summer months (December 2010–March 2011). Intracellular microcystins (MCs) were extracted using SPE C 18 cartridges, followed by separation, identification and quantification using LC-ESI-MS techniques. Quantitative variation studies of MCs were conducted with respect to MC congener isolated, sampling site and month. Three main MC congeners (MC-RR, -LR and-YR) were isolated, identified and quantified. In addition, three minor MCs (MC-WR, MC-(H 4 )YR and (D-Asp 3 , Dha 7 )MC-RR were also identified, but were not quantified. The MC dominance followed the order MC-RR>MC-LR>MC-YR across all sites and time. The maximum and minimum concentrations were 268 µg/g and 0.14 µg/g DW for MC-RR and MC-YR, respectively, of the total MCs quantified from this study. One-way ANOVA showed that there were no significant differences between average MC concentrations recorded across months ( P = 0.62), there was, however, a marginally-significant difference in concentrations among MC congeners ( P = 0.06). ANCOVA revealed a highly significant interaction between sites and MC congeners on MC concentration ( P

Keywords: summer; Hartbeespoort Dam; dry weight (DW); quantitative LC-ESI-MS; intracellular variations; microcystin-RR (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2012
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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