Genome Doubling of Northern Spicebush, Lindera benzoin L

Ramsey F. Arram, Thomas B. Morgan, John T. Nix, Yu-Lin Kao and Hsuan Chen ()
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Ramsey F. Arram: Department of Horticultural Science, North Carolina State University, 2721 Founders Dr, Raleigh, NC 27695, USA
Thomas B. Morgan: Department of Horticultural Science, North Carolina State University, 2721 Founders Dr, Raleigh, NC 27695, USA
John T. Nix: Mountain Crop Improvement Lab, Department of Horticultural Science, Mountain Horticultural Crops Research and Extension Center, North Carolina State University, 455 Research Drive, Mills River, NC 28759, USA
Yu-Lin Kao: Department of Life Sciences, National University of Kaohsiung, Kaohsiung 881, Taiwan
Hsuan Chen: Department of Horticultural Science, North Carolina State University, 2721 Founders Dr, Raleigh, NC 27695, USA

J, 2024, vol. 7, issue 2, 1-11

Abstract: Lindera benzoin is a dioecious understory shrub native to eastern North America. Northern spicebush is a beautiful shrub with a natural round shrub shape, golden-yellow fall foliage, attractive bright red drupes, and precocious yellow flowers in early spring; however, its market value as an ornamental value has been overlooked. To improve the ornamental values of this under-cultivated nursery crop, breeding for a better compact form, larger leaves, enlarged flower clusters and fruit, and increased stress tolerances could all be beneficial. Polyploidy manipulation is a valuable method to improve such traits for many ornamental plants. This study established the genome doubling method by oryzalin-infused solid agar treatment on young northern spicebush seedlings. The seedlings of two wild populations in North Carolina were collected and used. A total of 288 seedlings were treated with solid agar containing 150 µM oryzalin for 24, 72, and 120 h. The results were sporadic in their survival ratios and tetraploid conversion ratios between different treatments; however, a total of 16 tetraploid L. benzoin plants were produced in this study. The 24-h treatment showed the optimal result, with 7.1% of total treated seedlings or 15.2% of surviving seedlings converted into tetraploids. Tetraploid plants had visible differences in leaf morphology, a statistically significant enlarged stomata size, and reduced stomatal density compared to diploid plants. This research provides ploidy manipulation information for all future breeding processes of L. benzoin and related species.

Keywords: ploidy manipulation; polyploidy; autotetraploid; hybrid barrier; cytogenetics; autotetraploidization; native nursery crop; medicinal plant (search for similar items in EconPapers)
JEL-codes: I1 I10 I12 I13 I14 I18 I19 (search for similar items in EconPapers)
Date: 2024
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