Tagging Fluorescent Reporter to Epinecidin-1 Antimicrobial Peptide

Sivakumar Jeyarajan, Harini Priya Ramesh, Atchyasri Anbarasu, Jayasudha Jayachandran and Anbarasu Kumarasamy ()
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Sivakumar Jeyarajan: Microbial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India
Harini Priya Ramesh: Microbial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India
Atchyasri Anbarasu: Swamy Vivekananda Medical College Hospital and Research Institute, Tiruchengode 637205, Tamil Nadu, India
Jayasudha Jayachandran: Microbial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India
Anbarasu Kumarasamy: Microbial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India

J, 2025, vol. 8, issue 4, 1-15

Abstract: In this study, we successfully cloned the fluorescent proteins eGFP and DsRed in-frame with the antimicrobial peptide epinecidin-1 (FIFHIIKGLFHAGKMIHGLV) at the N-terminal. The cloning strategy involved inserting the fluorescent reporters into the expression vector, followed by screening for positive clones through visual fluorescence detection and molecular validation. The visually identified fluorescent colonies were confirmed as positive by PCR and plasmid migration assays, indicating successful cloning. This fusion of fluorescent reporters with a short antimicrobial peptide enables real-time visualization and monitoring of the peptide’s mechanism of action on membranes and within cells, both in vivo and in vitro. The fusion of eGFP and DsRed to epinecidin-1 did not impair the expression or fluorescence of the reporter protein.

Keywords: antimicrobial peptides; epinecidin-1; multi drug resistance; fluorescent reporter (search for similar items in EconPapers)
JEL-codes: I1 I10 I12 I13 I14 I18 I19 (search for similar items in EconPapers)
Date: 2025
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