Protein Hydrolysates from Flaxseed Oil Cake as a Media Supplement in CHO Cell Culture

Marijan Logarušić, Višnja Gaurina Srček, Sara Berljavac, Andreja Leboš Pavunc, Kristina Radošević and Igor Slivac
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Marijan Logarušić: Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
Višnja Gaurina Srček: Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
Sara Berljavac: Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
Andreja Leboš Pavunc: Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
Kristina Radošević: Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
Igor Slivac: Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia

Resources, 2021, vol. 10, issue 6, 1-12

Abstract: This is the first report about flaxseed protein hydrolysates applied as media supplements in CHO cell culture. The hydrolysates were produced by three separate enzymatic digestions of proteins isolated from flaxseed oil cake. The enzymes used were Alcalase , Neutrase , and Protamex , and the most efficient hydrolysis was achieved with Alcalase . The three hydrolysates were first tested as a partial substitute for serum in basal media in order to evaluate their effects on the adherent IgG-producing CHO cell line. The cells that grew in such media reached higher density than the cells in media supplemented with serum only. Consequently, the increased cell number improved the final IgG titer. In the next experiment, the impact of hydrolysates was evaluated in suspension CHO culture adapted to chemically defined media. In this preliminary investigation, the cells showed no response to the hydrolysate addition concerning the growth rate and productivity. Despite this outcome, we speculate that low molecular mass components in the hydrolysates, besides nutritive, may have a cell-protective function.

Keywords: flaxseed oil cake; protein hydrolysates; cell culture media supplementation; CHO cell technology (search for similar items in EconPapers)
JEL-codes: Q1 Q2 Q3 Q4 Q5 (search for similar items in EconPapers)
Date: 2021
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