Mutation Breeding of a N -methyl- N -nitrosourea (MNU)-Induced Rice ( Oryza sativa L. ssp. Indica ) Population for the Yield Attributing Traits

Tran Dang Xuan, Truong Thi Tu Anh, Hoang-Dung Tran, Tran Dang Khanh and Tran Dang Dat
Additional contact information
Tran Dang Xuan: Graduate School for International Development and Cooperation, Hiroshima University, Hiroshima 739-8529, Japan
Truong Thi Tu Anh: Graduate School for International Development and Cooperation, Hiroshima University, Hiroshima 739-8529, Japan
Hoang-Dung Tran: Department of Biotechnology, NTT Institute of Hi-Technology, Nguyen Tat Thanh University, Ho Chi Minh 72820, Vietnam
Tran Dang Khanh: Department of Genetic Engineering, Agricultural Genetics Institute, Hanoi 122300, Vietnam
Tran Dang Dat: Khai Xuan International Co. Ltd., Ha Dong District, Duong Noi Ward, LK20A-20B, Khai Xuan Building, Hanoi 152611, Vietnam

Sustainability, 2019, vol. 11, issue 4, 1-15

Abstract: Difficulties in breeding new rice cultivars that have a high yield, are acceptable quality, and are tolerant to environmental stresses have been the major constraint of rice production in many developing countries, as these traits are determined by multiple genes associated with complicated and uncontrollable gene segregations.Furthermore, the gene/QTL (quantitative trait locus) introduced to the cultivar is unstable due to the interaction among the active genes, which determine the phenotypic performance, not yet been well understood or controllable. In this study, the N -methyl- N -nitrosourea (MNU)-induced mutation was applied to the heterozygote of the F 1 generation from the cross between TBR 1 (female) and KD 18 (male parent). The phenotype and genotype of the M 2 and M 3 generations were evaluated and showed that the mutant population phenotypes, including the plant height, semi-dwarfism, amylose content, protein content, gel consistency, grain yield, and spikelet fertility, varied. Interestingly, no segregation among the genotypes in the M 2 and M 3 generations was observed, while the genotypes of the control population were either paternally inherited or indeterminable when using 28 polymorphism simple sequence repeat (SSR) markers that were identified on parental lines from 200 markers. The MNU-induced mutation caused maternal inheritance in the segregating populations, as primarily important agronomic traits were maternally succeeded from the female line TBR 1 . The findings of this study indicated that, through the use of MNU, the breeding of rice cultivars with close genetic backgrounds (similarity coefficient = 0.52) could be shortened by the maternal control of important qualities, such as pest and disease resistance and high yield, thus contributing to sustainable rice production for rice farmers. Further examination of rice cultivars with a greater difference in the genetic background should be subsequently conducted.

Keywords: MNU mutation; SSR marker; maternal inheritance; rice; important agronomic trait; rice breeding; sustainable rice production (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.mdpi.com/2071-1050/11/4/1062/pdf (application/pdf)
https://www.mdpi.com/2071-1050/11/4/1062/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jsusta:v:11:y:2019:i:4:p:1062-:d:206942

Access Statistics for this article

Sustainability is currently edited by Ms. Alexandra Wu

More articles in Sustainability from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().