Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with Real-Time PCR on Frass

Domenico Rizzo, Andrea Taddei, Daniele Da Lio, Francesco Nugnes, Eleonora Barra, Luciana Stefani, Linda Bartolini, Raffaele V. Griffo, Paola Spigno, Lucia Cozzolino, Elisabetta Rossi and Antonio P. Garonna
Additional contact information
Domenico Rizzo: Laboratory of Phytopathological Diagnostics and Molecular Biology, Plant Protection Service of Tuscany, 51100 Pistoia, Italy
Andrea Taddei: ANSES Plant Health Laboratory, Entomology and Invasive Plants Unit, 34988 Montferrier-sur-Lez CEDEX, France
Daniele Da Lio: Department of Agricultural, Food and Agro-Environmental Sciences, University of Pisa, 56124 Pisa, Italy
Francesco Nugnes: National Research Council (CNR), Institute for Sustainable Plant Protection, 80055 Portici, Italy
Eleonora Barra: Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
Luciana Stefani: Laboratory of Phytopathological Diagnostics and Molecular Biology, Plant Protection Service of Tuscany, 51100 Pistoia, Italy
Linda Bartolini: Laboratory of Phytopathological Diagnostics and Molecular Biology, Plant Protection Service of Tuscany, 51100 Pistoia, Italy
Raffaele V. Griffo: Plant Protection Service of Campania, Centro Direzionale, 80124 Napoli, Italy
Paola Spigno: Phytopathological Laboratory, Campania Region, 80141 Napoli, Italy
Lucia Cozzolino: Phytopathological Laboratory, Campania Region, 80141 Napoli, Italy
Elisabetta Rossi: Department of Agricultural, Food and Agro-Environmental Sciences, University of Pisa, 56124 Pisa, Italy
Antonio P. Garonna: Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy

Sustainability, 2020, vol. 12, issue 15, 1-12

Abstract: Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae), the red-necked longhorn beetle is native to eastern Asia, where it is an important wood-borer of fruit and ornamental species of the genus Prunus . A. bungii is a quarantine pest in the European Union, following its accidental introduction and establishment in Germany and Italy, and is currently included in the list of priority pests. To confirm its infestations in outbreak areas, adult or larval specimens are needed to perform morphological or molecular analyses. The presence of A. bungii larvae inside the attacked trees makes the collection of specimens particularly difficult. Thus, we present two diagnostic protocols based on frass analysis with real-time PCR (probe and SYBR Green). The results obtained show that a non-invasive approach for detecting the presence of this harmful invasive pest can be a reliable and accurate alternative diagnostic tool in phytosanitary surveys, as well as to outline a sustainable pest management strategy.

Keywords: frass DNA; non-invasive diagnostic tool; phytosanitary survey; quarantine pest; xylophagous insect (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2071-1050/12/15/6041/pdf (application/pdf)
https://www.mdpi.com/2071-1050/12/15/6041/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jsusta:v:12:y:2020:i:15:p:6041-:d:390797

Access Statistics for this article

Sustainability is currently edited by Ms. Alexandra Wu

More articles in Sustainability from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().