Response Surface Methodology Optimization and Kinetics of Diesel Degradation by a Cold-Adapted Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05

Mansur Abdulrasheed, Azham Zulkharnain, Nur Nadhirah Zakaria, Ahmad Fareez Ahmad Roslee, Khalilah Abdul Khalil, Suhaimi Napis, Peter Convey, Claudio Gomez-Fuentes and Siti Aqlima Ahmad
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Mansur Abdulrasheed: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Azham Zulkharnain: Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama 337-8570, Japan
Nur Nadhirah Zakaria: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Ahmad Fareez Ahmad Roslee: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Khalilah Abdul Khalil: School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
Suhaimi Napis: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Peter Convey: British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 0ET, UK
Claudio Gomez-Fuentes: Department of Chemical Engineering, Universidad de Magallanes, Avda. Bulnes, 01855 Punta Arenas, Región de Magallanes y Antártica Chilena, Chile
Siti Aqlima Ahmad: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Sustainability, 2020, vol. 12, issue 17, 1-14

Abstract: Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can be an effective and eco-friendly approach for their remediation. However, since the introduction of non-native organisms, including microorganisms, into the Antarctic or between the distinct biogeographical regions within the continent is not permitted under the Antarctic Treaty, it is crucial to discover native oil-degrading, psychrotolerant microorganisms that can be used in diesel bioremediation. The primary aim of the current study is to optimize the conditions for growth and diesel degradation activity of an Antarctic local bacterium, Arthrobacter sp. strain AQ5-05, using the Plackett-Burman approach and response surface method (RSM) via a central composite design (CCD) approach. Based on this approach, temperature, pH, and salinity were calculated to be optimum at 16.30 °C, pH 7.67 and 1.12% ( w / v ), respectively. A second order polynomial regression model very accurately represented the experimental figures’ interpretation. These optimized environmental conditions increased diesel degradation from 34.5% (at 10 °C, pH 7.00 and 1.00% ( w / v ) salinity) to 56.4%. Further investigation of the kinetics of diesel reduction by strain AQ5-05 revealed that the Teissier model had the lowest RMSE and AICC values. The calculated values for the Teissier constants of maximal growth rate, half-saturation rate constant for the maximal growth, and half inhibition constants ( μ max , Ks, and Ki), were 0.999 h −1 , 1.971% ( v/v ) and 1.764% ( v/v ), respectively. The data obtained therefore confirmed the potential application of this cold-tolerant strain in the bioremediation of diesel-contaminated Antarctic soils at low temperature.

Keywords: Antarctica; Arthrobacter; diesel bioremediation; growth kinetics; response surface methodology (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations: View citations in EconPapers (5)

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