Linking Organic Metabolites as Produced by Purpureocillium Lilacinum 6029 Cultured on Karanja Deoiled Cake Medium for the Sustainable Management of Root-Knot Nematodes

Abhishek Sharma, Aditi Gupta, Manu Dalela, Satyawati Sharma, R. Z. Sayyed, Hesham Ali El Enshasy and Elsayed Ahmed Elsayed
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Abhishek Sharma: Amity Food and Agriculture Foundation, Amity University Uttar Pradesh, Noida 201313, India
Aditi Gupta: Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
Manu Dalela: Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
Satyawati Sharma: Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
R. Z. Sayyed: Department of Microbiology, PSGVP Mandal’s Arts, Science, and Commerce College, Maharashtra 425409, India
Hesham Ali El Enshasy: Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Johor 81310, Malaysia
Elsayed Ahmed Elsayed: Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

Sustainability, 2020, vol. 12, issue 19, 1-9

Abstract: Root-knot nematodes pose a serious threat to agriculture and forest systems, causing significant losses of the crop worldwide. A wide range of chemical nematicides has traditionally been used to manage phyto-nematodes. However, due to their ill effects on the environment, biological control agents (BCAs) like Purpureocillium lilacinum that exhibit antagonistic effects on root-knot nematodes are preferred. The current study focused on identifying nematicidal metabolites produced by the fungus Purpureocillium lilacinum cultivated on akaranja deoiled cake-based liquid medium through bioactivity-guided fractionation against Meloidogyne incognita . Column chromatography of the ethyl acetate extract of fungal filtrate exhibited the most potent fraction (fraction 14–15), giving 94.6% egg mass hatching inhibition on the 5th day and a maximum nematicidal activity of 62% against second-stage juveniles after 48 h at 5000 mg/L. Gas chromatography coupled with mass spectrometry (GC-MS) analysis of this fraction revealed five major compounds, viz., 2-ethyl butyric acid, phenyl ethyl alcohol, benzoic acid, benzene acetic acid, and 3,5-Di-t-butylphenol. These biocompounds have potential biocontrol applications in agriculture, but further in vivo studies are warranted.

Keywords: bioassay; fourier transform infrared (FTIR); nematicidal; organic volatiles; scanning electron microscopy (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2020
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