Changes of Soil Microbes Related with Carbon and Nitrogen Cycling after Long-Term CO 2 Enrichment in a Typical Chinese Maize Field

Tiantian Diao, Zhengping Peng, Xiaoguang Niu, Rongquan Yang, Fen Ma and Liping Guo
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Tiantian Diao: Key Lab for Agro-Environment, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zhengping Peng: College of Resources and Environmental, Hebei Agricultural University, Baoding 071001, China
Xiaoguang Niu: Key Lab for Agro-Environment, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Rongquan Yang: Key Lab for Agro-Environment, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Fen Ma: Key Lab for Agro-Environment, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Liping Guo: Key Lab for Agro-Environment, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Sustainability, 2020, vol. 12, issue 3, 1-19

Abstract: Elevated atmospheric CO 2 concentration (eCO 2 ) has been the most important driving factor and characteristic of climate change. To clarify the effects of eCO 2 on the soil microbes and on the concurrent status of soil carbon and nitrogen, an experiment was conducted in a typical summer maize field based on a 10-year mini FACE (Free Air Carbon Dioxide Enrichment) system in North China. Both rhizospheric and bulk soils were collected for measurement. The soil microbial carbon (MBC), nitrogen (MBN), and soil mineral N were measured at two stages. Characteristics of microbes were assayed for both rhizospheric soil and bulk soils at the key stage. We examined the plasmid copy numbers, diversities, and community structures of bacteria (in terms of 16s rRNA), fungi (in terms of ITS-internal transcribed spacer), ammonia oxidizing bacteria (AOB) and denitrifiers including nirK, nirS, and nosZ using the Miseq sequencing technique. Results showed that under eCO 2 conditions, both MBC and MBN in rhizospheric soil were increased significantly. The quantity of ITS was increased in the eCO 2 treatment compared with that in the ambient CO 2 (aCO 2 ) treatment, while the quantity of 16s rRNA in rhizospheric soil showed decrease in the rhizospheric soil in the eCO 2 treatment. ECO 2 changed the relative abundance of microbes in terms of compositional proportion of some orders or genera particularly in the rhizospheric soil-n particular, Chaetomium increased for ITS, Subgroups 4 and 6 increased for 16s rRNA, Nitrosospira decreased for AOB, and some genera showed increase for nirS, nirK, and nosZ. Nitrate N was the main inorganic nitrogen form at the tasseling stage and both quantities of AOB and denitrifiers, as well as the nosZ/(nirS+nirK) showed an increase under eCO 2 conditions particularly in the rhizospheric soil. The Nitrosospira decreased in abundance under eCO 2 conditions in the rhizospheric soil and some genera of denitrifiers also showed differences in abundance. ECO 2 did not change the diversities of microbes significantly. In general, results suggested that 10 years of eCO 2 did affect the active component of C and N pools (such as MBC and MBN) and both the quantities and relative abundance of microbes which are involved in carbon and nitrogen cycling, possibly due to the differences in both the quantities and component of substrate for relevant microbes in the rhizospheric soils.

Keywords: rhizosphere soil; elevated CO 2; microbial community; nitrifier; denitrifier; ammonia oxidizing bacteria (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2020
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