The Production of Lipids Using 5-Hydorxymethy Furfural Tolerant Rhodotorula graminis Grown on the Hydrolyzates of Steam Pretreated Softwoods

Seiji Nakagame, Yuta Shimizu and Jack N. Saddler
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Seiji Nakagame: Department of Applied Bioscience, Kanagawa Institute of Technology, 1030 Shimo-ogino, Atsugi, Kanagawa 243-0292, Japan
Yuta Shimizu: Department of Applied Bioscience, Kanagawa Institute of Technology, 1030 Shimo-ogino, Atsugi, Kanagawa 243-0292, Japan
Jack N. Saddler: Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC V6T1Z4, Canada

Sustainability, 2020, vol. 12, issue 3, 1-9

Abstract: Acid catalyzed (SO 2 ) steam pretreated softwoods inevitably contain furans such as 5-hydorxymethy furfural (HMF) and furfural, which are derived from the respective degradation of component hexoses and pentoses. As these materials are known to be inhibitory to fermentation, six oleaginous yeasts were grown on corn steep liquor (CSL) medium containing HMF (0.2%) and furfural (0.1%) to assess their resistance to possible inhibition and its possible influence on lipid production. R. graminis showed the highest tolerance to HMF (0.2%) and furfural (0.1%) when they were added individually to the CSL medium. However, when both HMF (0.2%) and furfural (0.1%) were added together, this inhibited the growth of R. graminis . Subsequent evaporation of the CSL medium successfully removed furfural from the CSL medium and increased the sugar concentration. However, the residual concentration of HMF (0.4%) still inhibited R. graminis growth. To try to improve HMF tolerance, R. graminis was slowly acclimatized in medium containing HMF (0.4%) and was eventually able to produce 1.8 g/L of lipids after four days of growth in the HMF containing medium. This was close to the same amount of lipid produced as when R. graminis was grown in the CSL medium without HMF and furfural. This indicated that an acclimatization strategy is a promising way to enhance lipids production when R. graminis is grown on the hydrolyzates of SO 2 -catalyzed steam pretreated lignocellulosic substrates.

Keywords: SO 2 -catalyzed steam pretreatment; Rhodotorula graminis; HMF tolerant strain (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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