Production, Purification, and Characterization of Bacillibactin Siderophore of Bacillus subtilis and Its Application for Improvement in Plant Growth and Oil Content in Sesame

S. Nithyapriya, Sundaram Lalitha, R. Z. Sayyed, M. S. Reddy, Daniel Joe Dailin, Hesham A. El Enshasy, Ni Luh Suriani and Susila Herlambang
Additional contact information
S. Nithyapriya: Department of Botany, Periyar University, Salem, Tamil Nadu 636011, India
Sundaram Lalitha: Department of Botany, Periyar University, Salem, Tamil Nadu 636011, India
R. Z. Sayyed: Department of Microbiology, PSGVP Mandal’s Arts, Science and Commerce College, Shahada, Maharashtra 425409, India
M. S. Reddy: Asian PGPR Society for Sustainable Agriculture, Auburn University, Auburn, AL 36830, USA
Daniel Joe Dailin: Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru 81310, Malaysia
Hesham A. El Enshasy: Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru 81310, Malaysia
Ni Luh Suriani: Biology Study Program, Mathematics and Natural Sciences Faculty, Udayana University, Bali 80361, Indonesia
Susila Herlambang: Department of Soil Science Faculty of Agriculture, Universitas Pembangunan Nasional Veteran, Yogyakarta 55293, Indonesia

Sustainability, 2021, vol. 13, issue 10, 1-18

Abstract: Siderophores are low molecular weight secondary metabolites produced by microorganisms under low iron stress as a specific iron chelator. In the present study, a rhizospheric bacterium was isolated from the rhizosphere of sesame plants from Salem district, Tamil Nadu, India and later identified as Bacillus subtilis LSBS2. It exhibited multiple plant-growth-promoting (PGP) traits such as hydrogen cyanide (HCN), ammonia, and indole acetic acid (IAA), and solubilized phosphate. The chrome azurol sulphonate (CAS) agar plate assay was used to screen the siderophore production of LSBS2 and quantitatively the isolate produced 296 mg/L of siderophores in succinic acid medium. Further characterization of the siderophore revealed that the isolate produced catecholate siderophore bacillibactin. A pot culture experiment was used to explore the effect of LSBS2 and its siderophore in promoting iron absorption and plant growth of Sesamum indicum L. Data from the present study revealed that the multifarious Bacillus sp. LSBS2 could be exploited as a potential bioinoculant for growth and yield improvement in S. indicum .

Keywords: bacillibactin; bioinoculant; P solubilization; plant growth promotion; Sesamum indicum; siderophore (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.mdpi.com/2071-1050/13/10/5394/pdf (application/pdf)
https://www.mdpi.com/2071-1050/13/10/5394/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jsusta:v:13:y:2021:i:10:p:5394-:d:552859

Access Statistics for this article

Sustainability is currently edited by Ms. Alexandra Wu

More articles in Sustainability from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().