Galactomannans for Entrapment of Gliomastix murorum Laccase and Their Use in Reactive Blue 2 Decolorization

Itzel C. Romero-Soto, Raúl B. Martínez-Pérez, Jorge A. Rodríguez, Rosa M. Camacho-Ruiz, Alejandra Barbachano-Torres, Martha Martín del Campo, Juan Napoles-Armenta, Jorge E. Pliego-Sandoval, María O. Concha-Guzmán and María Angeles Camacho-Ruiz
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Itzel C. Romero-Soto: Laboratorio de Investigación en Biotecnología, Centro Universitario del Norte, Universidad de Guadalajara, Colotlán 46200, Jalisco, Mexico
Raúl B. Martínez-Pérez: Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Zapopan 45019, Jalisco, Mexico
Jorge A. Rodríguez: Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Zapopan 45019, Jalisco, Mexico
Rosa M. Camacho-Ruiz: Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Zapopan 45019, Jalisco, Mexico
Alejandra Barbachano-Torres: Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Zapopan 45019, Jalisco, Mexico
Martha Martín del Campo: Laboratorio de Investigación en Biotecnología, Centro Universitario del Norte, Universidad de Guadalajara, Colotlán 46200, Jalisco, Mexico
Juan Napoles-Armenta: Facultad de Agronomía, Universidad Autónoma de Nuevo León, Francisco I. Madero S/N, Ex Hacienda el Cañada, Gral. Escobedo 66050, Nuevo León, Mexico
Jorge E. Pliego-Sandoval: Laboratorio de Ciencias de la Naturaleza. Centro Universitario del Sur, Av. Enrique Arreola Silva 883, Col Centro, Ciudad Guzmán 49000, Jalisco, Mexico
María O. Concha-Guzmán: Laboratorio de Investigación en Biotecnología, Centro Universitario del Norte, Universidad de Guadalajara, Colotlán 46200, Jalisco, Mexico
María Angeles Camacho-Ruiz: Laboratorio de Investigación en Biotecnología, Centro Universitario del Norte, Universidad de Guadalajara, Colotlán 46200, Jalisco, Mexico

Sustainability, 2021, vol. 13, issue 16, 1-12

Abstract: In the present study, a novel laccase from ascomycete Gliomastix murorum was produced in agro-industrial wastes and entrapped in galactomannan beads for Reactive Blue 2 (Rb-2) decolorization. The maximum laccase production in agave bagasse-based medium occurred at 72 h (1798.6 UL −1 ). Entrapped laccase decolorized ˃80% of 0.5 mM Rb-2 in 2 h without the addition of redox mediator. K m for Rb-2 substrate was 1.42 mM, with a V max of 1.19 µmol min −1 for entrapped laccase. Galactomannan matrices produce stability to acid pH (2–5) and temperatures from 20–70 °C. Reusability assays showed that entrapped laccase could retain efficient Rb-2 decolorization of ˃80% six times. In general, galactomannan used for entrapment of laccase provides economic advantages in large-scale wastewater treatment due to its natural origin and efficient results.

Keywords: galactomannan beads; ascomycete; laccase; dye decolorization; cibacron blue 3GA; immobilization (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2021
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