Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

Peyman Abdeshahian, Jesús Jiménez Ascencio, Rafael R. Philippini, Felipe Antonio Fernandes Antunes, Avinash P. Ingle, Mojgan Abdeshahian, Júlio César dos Santos and Silvio Silvério da Silva
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Peyman Abdeshahian: Department of Biotechnology, Engineering School of Lorena, University of São Paulo, São Paulo 12602-810, Brazil
Jesús Jiménez Ascencio: Department of Biotechnology, Engineering School of Lorena, University of São Paulo, São Paulo 12602-810, Brazil
Rafael R. Philippini: Department of Biotechnology, Engineering School of Lorena, University of São Paulo, São Paulo 12602-810, Brazil
Felipe Antonio Fernandes Antunes: Department of Biotechnology, Engineering School of Lorena, University of São Paulo, São Paulo 12602-810, Brazil
Avinash P. Ingle: Biotechnology Centre, Department of Agricultural Botany, Panjabrao Deshmukh Krishi Vidyapeeth, Akola 444104, India
Mojgan Abdeshahian: Department of Agriculture Science, Payame Noor University (PNU), Tehran 19395-4697, Iran
Júlio César dos Santos: Department of Biotechnology, Engineering School of Lorena, University of São Paulo, São Paulo 12602-810, Brazil
Silvio Silvério da Silva: Department of Biotechnology, Engineering School of Lorena, University of São Paulo, São Paulo 12602-810, Brazil

Sustainability, 2021, vol. 13, issue 17, 1-16

Abstract: Lasiodiplodan is a β-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae , using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v / v ) and sodium hydroxide (1% w / v ), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 °C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL −1 of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg −1 glucose consumed and 0.016 gL −1 h −1 , respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a β-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan.

Keywords: lasiodiplodan; sugarcane straw; Lasiodiplodia theobromae CCT3966; fermentation process (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2021
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