Isolation, Characterization and Identification of a New Lysinibacillus fusiformis Strain ZC from Metlaoui Phosphate Laundries Wastewater: Bio-Treatment Assays

Ines Taieb, Sonia Ben Younes, Boutheina Messai, Sami Mnif, Ridha Mzoughi, Amina Bakhrouf, Chédia Jabeur, Juan Alfonso Ayala Serrano and Ali Ellafi
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Ines Taieb: Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia
Sonia Ben Younes: Faculté des Sciences de Gafsa, Campus Universitaire Sidi Ahmed Zarroug, Université de Gafsa, Gafsa 2112, Tunisia
Boutheina Messai: Faculté des Sciences de Gafsa, Campus Universitaire Sidi Ahmed Zarroug, Université de Gafsa, Gafsa 2112, Tunisia
Sami Mnif: Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, P.O. Box 1177, Sfax 3018, Tunisia
Ridha Mzoughi: Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia
Amina Bakhrouf: Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia
Chédia Jabeur: Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia
Juan Alfonso Ayala Serrano: Centro de Biologia Molecular “Severo Ochoa”, Campus Cantoblanco, CSIC—Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain
Ali Ellafi: Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia

Sustainability, 2021, vol. 13, issue 18, 1-19

Abstract: The aim of the present study is to isolate, characterize and identify a novel strain ZC from the Metlaoui phosphate laundries wastewater (MPLW). The chemical characterization of this phosphate rich effluent showed an alkaline pH and is saline, highly turbid and rich in suspended matter and total solids. The MPLW samples were loaded with potentially toxic metals, presented in decreasing order as follows: magnesium (5655 mg L −1 ), potassium (45 mg L −1 ), lead (1 mg L −1 ), iron (0.7 mg L −1 ), cadmium (0.5 mg L −1 ), copper (0.3 mg L −1 ) and zinc (0.1 mg L −1 ). Due to the high COD/BOD 5 ratio, a poorly biodegradable organic load is underlining. The newly isolated strain was identified as Lysinibacillus fusiformis using 16S rDNA sequencing analysis. The viability of this new strain was tested in presence of the zinc, lead, cadmium, manganese and copper at 1, 10 and 100 mM. The L. fusiformis survival, under metallic stress, was inversely proportional to metal ion concentrations, while lead and zinc were the most toxic ones using MTT assay. Then, the newly isolated strain was characterized in terms of enzyme production, proteomic alteration and antibiotic resistance. The strain ZC revealed some modifications in the biochemical and enzymatic profiles by either the appearance or/and the disappearance of some activities. In addition, the increase in metal ions stress and concentrations was proportional to the adherence and to the hydrophobicity. The presence of the metal ions suggested the change of sensitivity to the resistance of this strain towards tobramycin, kanamycin, neomycin, netilmicin and cefoxitin, showing an increase in the MAR index . The strain ZC, used as a biological tool for MPLW treatment, showed a reduction in the metal ion contents. This reduction was due to accumulation and/or adsorption, showing a bioprocessing performance of the newly isolated L. fusiformis .

Keywords: Lysinibacillus fusiformis; phosphate laundries wastewater; heavy metal ions; resistance; stress (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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