Antibiotic Resistance of Escherichia coli Isolated from Processing of Brewery Waste with the Addition of Bulking Agents

Katarzyna Wolny-Koładka and Marek Zdaniewicz
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Katarzyna Wolny-Koładka: Department of Microbiology and Biomonitoring, University of Agriculture in Krakow, Mickiewicza Ave 24/28, 30-059 Krakow, Poland
Marek Zdaniewicz: Department of Fermentation Technology and Microbiology, University of Agriculture in Krakow, Balicka Street 122, 30-149 Krakow, Poland

Sustainability, 2021, vol. 13, issue 18, 1-10

Abstract: The aim of the study was to determine the drug resistance profile and to assess the presence of genes responsible for the production of extended-spectrum beta-lactamases in Escherichia coli isolated from energy-processed hop sediment with the addition of bulking agents. Antibiotic resistance was determined by the disk diffusion method and the PCR technique to detect genes determining the extended-spectrum beta-lactamases (ESBLs) mechanism. A total of 100 strains of E. coli were collected. The highest resistance was found to aztreonam, tetracycline, ampicillin, ticarcillin, and ceftazidime. The bacteria collected were most often resistant to even 10 antibiotics at the same time and 15 MDR strains were found. The ESBL mechanism was determined in 14 isolates. Among the studied genes responsible for beta-lactamase production, blaTEM was the most common (64%). The study revealed that the analysed material was colonised by multi-drug-resistant strains of E. coli , which pose a threat to public health. The obtained results encourage further studies to monitor the spread of drug resistance in E. coli .

Keywords: Escherichia coli; extended-spectrum beta-lactamases; brewing; hot trub; bulking agents (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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