Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China

Meng Wang, Ling Wang, Qian Li, Hang Liu, Yuan Lin and Lichun Wang
Additional contact information
Meng Wang: Institute of Agricultural Environment and Resources Research, Jilin Academy of Agricultural Sciences, Changchun 130033, China
Ling Wang: Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, College of Resource and Environmental Science, Jilin Agricultural University, Changchun 130118, China
Qian Li: Institute of Agricultural Environment and Resources Research, Jilin Academy of Agricultural Sciences, Changchun 130033, China
Hang Liu: Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, College of Resource and Environmental Science, Jilin Agricultural University, Changchun 130118, China
Yuan Lin: Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, College of Resource and Environmental Science, Jilin Agricultural University, Changchun 130118, China
Lichun Wang: Institute of Agricultural Environment and Resources Research, Jilin Academy of Agricultural Sciences, Changchun 130033, China

Sustainability, 2021, vol. 13, issue 21, 1-14

Abstract: The soil nitrogen (N) cycle is an essential role of the biogeochemical cycle. Bacteria play an irreplaceable part in the soil N cycle, but the impact of different N gradients on bacterial communities remains unclear. The purpose of this research was to explore the bacterial abundance, community composition, and diversity under different N application rates in a water-limited area. We investigated the bacterial abundance, diversity, community composition, and structure under five different N gradients (0, 90, 150, 210, and 270 kg ha −1 ) using real-time quantitative PCR and high-throughput sequencing, and then explored bacterial functional groups with FAPROTAX. N application significantly affected bacterial abundance and community composition. Bacterial diversity was enhanced at low N application rates and reduced at higher N application rates. Principal coordinate analysis showed that bacterial community structure was separated into two groups between low N application rates and high N application rates; these differences in bacterial community structure may be driven by available nitrogen (AN). The results of FAPROTAX revealed that N application promoted the functions of Aerobic_nitrite_oxidation, Nitrate_reduction, and Aerobic_ammonia_oxidation, but inhibited the Nitrogen_fixation function of the bacterial community. The high N network caused the reduction of network structure stability. Our results revealed that N fertilizer driven bacterial community structure and soil nutrients were the main influential factors in the variation of bacterial community structure. We suggest that the optimal N application rate in this study may be approximately 150 kg ha −1 , based on the variations of soil properties and bacterial community structure in semi-arid areas.

Keywords: bacterial community; Illumina MiSeq sequencing; nitrogen fertilization; soil properties (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2071-1050/13/21/11967/pdf (application/pdf)
https://www.mdpi.com/2071-1050/13/21/11967/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jsusta:v:13:y:2021:i:21:p:11967-:d:667876

Access Statistics for this article

Sustainability is currently edited by Ms. Alexandra Wu

More articles in Sustainability from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().