Screening Technique Based on Seed and Early Seedling Parameters for Cold Tolerance of Selected F 2 -Derived F 3 Rice Genotypes under Controlled Conditions

Javeed Lone, Asif Shikari, Najeeb Sofi, Sajad Ganie, Manmohan Sharma, Mamta Sharma, Mukul Kumar, Muhammad Hamzah Saleem, Khalid S. Almaary, Mohamed S. Elshikh, Yheni Dwiningsih and Muhammad Ammar Raza
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Javeed Lone: Mountain Research Centre for Field Crops, Khudwani, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar 192101, Jammu and Kashmir, India
Asif Shikari: Mountain Research Centre for Field Crops, Khudwani, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar 192101, Jammu and Kashmir, India
Najeeb Sofi: Mountain Research Centre for Field Crops, Khudwani, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar 192101, Jammu and Kashmir, India
Sajad Ganie: Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar 190025, Jammu and Kashmir, India
Manmohan Sharma: School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Chatha Jammu, Srinagar 180001, Jammu and Kashmir, India
Mamta Sharma: School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Chatha Jammu, Srinagar 180001, Jammu and Kashmir, India
Mukul Kumar: Department of Biochemistry and Crop Physiology, Bihar Agriculture University, Sabour Bhagalpur 813210, Bihar, India
Muhammad Hamzah Saleem: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Khalid S. Almaary: Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Mohamed S. Elshikh: Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Yheni Dwiningsih: Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, USA
Muhammad Ammar Raza: Key Laboratory of Fruits and Vegetables, School of Food Science and Biotechnolog, Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou 310018, China

Sustainability, 2022, vol. 14, issue 14, 1-11

Abstract: The cold tolerance studies were carried out in a bi-parental F 2 population of a cross between tolerant and susceptible parents (SKUA-529 and HEERA, respectively). The purpose was to screen the individuals of a population for primary cold-tolerance-related attributes. The information generated has a direct application and use in identifying cold tolerance quantitative trait loci (QTLs) and further can be used for genotyping with an appropriate marker system. The screening was carried out on F 2 -derived F 3 seeds and F 3 plants for seedling and agronomic traits, respectively. Two tests measuring cold tolerance were conducted. In experiment I, seeds were germinated for 28 days at 13 °C and 7 days at 28 °C, and in experiment II, the seeds were germinated for 72 h at 28 °C, 96 h at 13 °C, and once more for 72 h at 28 °C. Coleoptile length, germination percentage, and radical reduction percentages were all measured in experiment I. The radicle and coleoptile regeneration in experiment II were measured after the cold period. The improvement in cold tolerance was achieved through radicle regrowth, as evidenced by the difference between the second and first measurements. The individual lines from the F 2:3 population that recorded high germination (%) were #21, #13, #14, and #15. The percentage of coleoptile length (PERCOL %) was observed to be between the ranges of 23.33% to 53.00%. The reduction in coleoptile length (REDCOL %) was also obtained, and there was less reduction in #15, #16, and #14 and it had a range between 38.46% and 75%. Radicle regrowth (REDRAG) was high at 13 °C in #7, #11, #30, #35, and #36. Survival of the seedling range was between 33.33% and up to 100%, and the highest survival rate was observed in #16. The main objective of this rotation in temperature was to emulate field conditions where there has been a drop in temperature. The evaluations were done for primary cold stress tolerance traits, and it was found that most of these traits exhibited high variability. The mapping population developed may be utilized to generate a linkage map and locate QTLs for tolerance to cold stress in rice. Further, the identified donors for cold tolerance may be utilized for breeding programs aimed at the transfer of low-temperature stress tolerance into susceptible backgrounds. In general, a genotype with improved seedling germination rates, growth rates, and leaf yellowing scores; high seedling survival; lesser reduction in coleoptile length and in radicle development; and recovery following a cold shock at the seedling stage demonstrated its cold resistance. Genotypes with a low germination percentage, a greater number of days to germination, slow growth rate and higher leaf yellowing score, high reduction in coleoptile and radicle growth, and reduced seedling survival indicated cold susceptibility.

Keywords: radicle growth; seedling survival; coleoptile length; cold tolerance; germination rate (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
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