Sustainable Production of Biodiesel Using UV Mutagenesis as a Strategy to Enhance the Lipid Productivity in R. mucilaginosa

Joseph Antony Sundarsingh Tensingh and Vijayalakshmi Shankar
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Joseph Antony Sundarsingh Tensingh: School of Bioscience and Technology, CO 2 Research and Green Technologies Centre, VIT University, Vellore 632014, India
Vijayalakshmi Shankar: School of Bioscience and Technology, CO 2 Research and Green Technologies Centre, VIT University, Vellore 632014, India

Sustainability, 2022, vol. 14, issue 15, 1-15

Abstract: The future of petroleum-based fuel is biodiesel. Biodiesel is an eco-friendly fuel that can be used in any diesel engine without any alterations. Researchers have focused on biodiesel that can be produced from microbial lipids extracted from high lipid-yielding microbes. In this study, microbial cultures were screened for high lipid-yielding capabilities and mutated using UV radiation at three different time intervals of 30, 75, and 90 min. The Nile red fluorescence method was used to analyze high lipid-yielding microbes. An outstanding increase in biomass and lipid productivity was noted when the microbes were exposed to UV for 30 min. For example, an M30-8 UV-mutated strain produced a lipid yield of 68.5%. The lipids produced from the wild and mutated strains were analyzed using GCMS and FTIR spectrophotometric analysis. Then, the lipids extracted from both wild VS3 and UV-mutated M30-8 strains were transesterified using a base catalyst and the produced biodiesel was analyzed using ASTM standards. The aim and objective of the research was to mutate high lipid-yielding microbes by using UV radiation and produce biodiesel from the lipids extracted from both wild and UV-mutated strains.

Keywords: micro-organism; UV mutation; lipid extraction; GCMS; FTIR (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
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