Experimental Study on the Preparation of a Highly Active Bacterial Suspension for MICP in the South China Sea

Yuxuan Zhou, Jun Hu, Yuwei Wu (), Hui Zeng, Dongling Zeng and Zhixin Wang
Additional contact information
Yuxuan Zhou: School of Civil Engineering and Architecture, Hainan University, Haikou 570228, China
Jun Hu: School of Civil Engineering and Architecture, Hainan University, Haikou 570228, China
Yuwei Wu: School of Civil Engineering and Architecture, Hainan University, Haikou 570228, China
Hui Zeng: School of Civil Engineering and Architecture, Wuyi University, Jiangmen 529020, China
Dongling Zeng: Hainan Investigation Institute of Hydrogeology and Engineering Geology, Haikou 570206, China
Zhixin Wang: Hainan Investigation Institute of Hydrogeology and Engineering Geology, Haikou 570206, China

Sustainability, 2022, vol. 14, issue 15, 1-16

Abstract: Most previous studies investigating the use of microbial-induced calcium carbonate precipitation (MICP) to reinforce foundations have indicated that the final curing effect can be improved by changing the nutrient environment parameters during the MICP reaction. However, using such methods to improve the construction process increases the construction cost and the impact on the surrounding environment. This study was conducted to determine if we could prepare a bacterial suspension with high activity in a short period of time by examining the effects of inoculation time, the concentration of the inoculated bacterial suspension, and shaker speed during expansion to determine whether sporosarcina pasteurii would vigorously grow. Based on the analysis of the pattern of activity variations in the bacterial suspension, the most appropriate growth scheme for preparing high-activity bacterial suspensions when using MICP to strengthen structures in the South China Sea was proposed. In terms of the results, it was found that the trend of changes in activity and the peak time of maximum activity in the bacteriophage cultured under low-speed conditions in the expanded culture tended to be the same. The value of the bacteriophage’s activity was low. During medium speed culture of the bacterial suspension, urease activity peaked much higher than that of the other comparison groups, with the medium speed bacterial broth having the highest peak. As a result of the prolonged shaking incubation time, the effect of prolonged shaking on urease activity in the bacterial suspension was mainly reflected in the fact that the activity decay cycle of the colony itself was slowed.

Keywords: South China Sea environment; microbial-induced carbonate precipitation; training program; urease activity; change rule (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.mdpi.com/2071-1050/14/15/9748/pdf (application/pdf)
https://www.mdpi.com/2071-1050/14/15/9748/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jsusta:v:14:y:2022:i:15:p:9748-:d:882866

Access Statistics for this article

Sustainability is currently edited by Ms. Alexandra Wu

More articles in Sustainability from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().