Phytochemical Analysis Using cLC-DAD, Nutritional Importance and Assessment of Antioxidant, Antidiabetic and Anticholinesterase Activities of Ruta tuberculata Forssk Organic Extracts

Asma Saidi, Leila Hambaba, Mohamed Sabri Bensaad, Imed Eddine Kadi, Chawki Bensouici, Rokayya Sami, Sarah Alharthi, Abeer Elhakem, Zayed D. Alsharari, Roua S. Baty, Raja Saad Alruwais, Mahmood D. Aljabri, Mohammed M. Rahman, Ali A. Shati and Fahmy G. Elsaid
Additional contact information
Asma Saidi: Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMBPC), Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences, University Batna 2, Batna 05078, Algeria
Leila Hambaba: Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMBPC), Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences, University Batna 2, Batna 05078, Algeria
Mohamed Sabri Bensaad: Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMBPC), Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences, University Batna 2, Batna 05078, Algeria
Imed Eddine Kadi: Biotechnology Research Center, Ali Mendjli UV 03, Constantine 25000, Algeria
Chawki Bensouici: Biotechnology Research Center, Ali Mendjli UV 03, Constantine 25000, Algeria
Rokayya Sami: Department of Food Science and Nutrition, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Sarah Alharthi: Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Abeer Elhakem: Department of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam bin Abdulaziz University, P.O. Box 11942, Al-Kharj 16273, Saudi Arabia
Zayed D. Alsharari: Department of Clinical Nutrition, Faculty of Applied Medical Sciences, University of Tabuk, P.O. Box 4279, Tabuk 71491, Saudi Arabia
Roua S. Baty: Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Raja Saad Alruwais: Chemistry Department, Faculty of Science and Humanities, Shaqra University, P.O. Box 33, Dawadmi 17452, Saudi Arabia
Mahmood D. Aljabri: Department of Chemistry, University College in Al-Jamoum, Umm Al-Qura University, P.O. Box 715, Makkah 21955, Saudi Arabia
Mohammed M. Rahman: Department of Chemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia
Ali A. Shati: Biology Department, Science College, King Khalid University, P.O. Box 960, Abha 61421, Saudi Arabia
Fahmy G. Elsaid: Biology Department, Science College, King Khalid University, P.O. Box 960, Abha 61421, Saudi Arabia

Sustainability, 2022, vol. 14, issue 16, 1-24

Abstract: Among the Mediterranean aromatic plants, Ruta tuberculata Forssk. (Rutaceae) has been widely used as a traditional natural remedy against various disorders resulting from its divers’ pharmacological virtues. The aim of this study is to characterize for the first time the phenolic profile of its ethyl acetate (EtOAcE) and acetonic (AcE) extracts and to screen their in vitro antioxidant, antidiabetic, and neuroprotective activities. Phenolic content was determined using spectrophotometric and cLC-DAD analysis. Pharmacologically, in vitro antioxidant power was evaluated using six different antioxidant methods. Moreover, the antidiabetic and neuroprotective capacities were assessed in vitro by determining the α-amylase, α-glucosidase, and acetylcholinesterase inhibitory activities. Phytochemically, the highest flavonoid content was found in EtOAcE where the major identified compounds were myrecetin, rutin, sylimarin, naringenin, and quercetin. In presence of other phenolic acids, gallic acid was exclusively detected in AcE. Furthermore, both R. tuberculata extracts showed significantly remarkable antioxidant activities, especially the EtOAcE. Interestingly, AcE strongly inhibited the acetylcholinesterase and α-glucosidase, with the respective IC 50 values of 20.48 ± 0.2 and 104.5 ± 1.8 µg/mL. In this study, we also reported the nutritional quality associated with the identified phytocompounds. R. tuberculata organic extracts may offer exciting reserves to achieve new anti-diabetic and anti-Alzheimer drugs which have also antioxidant potential.

Keywords: Ruta tuberculata; antioxidant; anti-α-amylase; anti-α-glucosidase; anticholinesterase; phenolic analysis (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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