Antifungal, Antiviral, and HPLC Analysis of Phenolic and Flavonoid Compounds of Amphiroa anceps Extract

Emad H. El-Bilawy, Al-Naji A. Al-Mansori, Seham A. Soliman, Fatimah O. Alotibi, Abdulaziz A. Al-Askar, Amr A. Arishi, Abd El-Naser Sabry, Mohsen Mohamed Elsharkawy, Ahmed A. Heflish, Said I. Behiry and Ahmed Abdelkhalek ()
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Emad H. El-Bilawy: Faculty of Basic Science, King Salman International University, El Tor 46612, Egypt
Al-Naji A. Al-Mansori: Botany Department, Faculty of Science, Derna University, Derna 99999, Libya
Seham A. Soliman: Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City 21934, Egypt
Fatimah O. Alotibi: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Abdulaziz A. Al-Askar: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Amr A. Arishi: School of Molecular Sciences, The University of Western Australia, Perth, WA 6009, Australia
Abd El-Naser Sabry: Department of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta City 34517, Egypt
Mohsen Mohamed Elsharkawy: Department of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt
Ahmed A. Heflish: Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt
Said I. Behiry: Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt
Ahmed Abdelkhalek: Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City 21934, Egypt

Sustainability, 2022, vol. 14, issue 19, 1-13

Abstract: The increasing use of chemical control agents and pesticides to prevent plant disease has resulted in several human and environmental health problems. Seaweeds, e.g., Amphiroa anceps extracts, have significant antimicrobial activities against different human pathogens. However, their anti-phytopathogenic activities are still being investigated. In the present investigation, three fungal isolates were isolated from root rot and grey mold symptomatic strawberry plants and were molecularly identified by ITS primers to Fusarium culmorum , Rhizoctonia solani , and Botrytis cinerea with accession numbers MN398396, MN398398, and MN398400, respectively. In addition, the organic extract of the red alga Amphiroa anceps was assessed for its antifungal activity against the three identified fungal isolates and tobacco mosaic virus (TMV) infection. At 100 µg/mL, the A. anceps extract had the best biological activity against R. solani , B. cinerea , and TMV infection, with inhibition rates of 66.67%, 40.61%, and 81.5%, respectively. Contrarily, the A. anceps extract exhibited lower activity against F. culmorum , causing inhibition in the fungal mycelia by only 4.4% at the same concentration. The extract’s HPLC analysis revealed the presence of numerous phenolic compounds, including ellagic acid and gallic acid, which had the highest concentrations of 19.05 and 18.36 µg/mL, respectively. In this line, the phytochemical analysis also showed the presence of flavonoids, with the highest concentration recorded for catechin at 12.45 µg/mL. The obtained results revealed for the first time the effect of the A. anceps extract against the plant fungal and viral pathogens, making the seaweed extract a promising source for natural antimicrobial agents.

Keywords: Amphiroa anceps; seaweed; root rot; grey mold; antifungal; ITS; antiviral; TMV; extract; HPLC (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
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