Laccase-Oriented Immobilization Using Concanavalin A as an Approach for Efficient Glycoproteins Immobilization and Its Application to the Removal of Aqueous Phenolics

Abdelmageed M. Othman, Angeles Sanroman () and Diego Moldes
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Abdelmageed M. Othman: Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Buhouth St., Dokki, Giza 12622, Egypt
Angeles Sanroman: CINTECX, Department of Chemical Engineering, Universidade de Vigo, Campus Universitario as Lagoas–Marcosende, 36310 Vigo, Spain
Diego Moldes: CINTECX, Department of Chemical Engineering, Universidade de Vigo, Campus Universitario as Lagoas–Marcosende, 36310 Vigo, Spain

Sustainability, 2022, vol. 14, issue 20, 1-20

Abstract: An expanding number of human activities are contributing to the rising levels of aromatic compounds, which pose a major threat to the ecosystem. However, readily available microbial enzymes might be used to remediate contaminated wastewater in an economical and environmentally benign manner. In this study, an efficient method of laccase-oriented immobilization on modified Immobead 150P was proposed. The oriented immobilization technique using aminated laccase exceeds in both protein loading onto the carrier (4.26 mg/g) and immobilization yield (93.57%) due to the availability of more active sites. The oriented aminated laccase preserves 100% and 95% of its original activity after six and ten cycles of operation, respectively. The thermal stability performance of the oriented enzyme was the best among both free and random immobilized forms, since it was able to conserve 79% and 44% of its initial activity after 6 h at 50 °C and 60 °C, respectively. The ideal pH of oriented immobilized laccase was altered from 3.0 to 4.0, and it was more stable than both free and random immobilized laccases at pH 7.0. Finally, the integration of the adsorption capacity of Immobead 150P and the biodegradation ability of laccase promises the efficient removal of aqueous phenolics. Oriented immobilized laccase may provide a significant new approach for wastewater treatment, according to these findings.

Keywords: laccase; oriented immobilization; concanavalin A; Immobead 150P; phenolics removal (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
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