Evaluation of Aurantiochytrium mangrovei Biomass Grown on Digestate as a Sustainable Feed Ingredient of Sea Bass, Dicentrarchus labrax, Juveniles and Larvae

Philippe Soudant (), Mariana Ventura, Luc Chauchat, Maurean Guerreiro, Margaux Mathieu-Resuge, Fabienne Le Grand, Victor Simon, Sophie Collet, José-Luis Zambonino-Infante, Nelly Le Goïc, Christophe Lambert, Fleuriane Fernandes, Alla Silkina, Marcella Fernandes de Souza and Denis de la Broise
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Philippe Soudant: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Mariana Ventura: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Luc Chauchat: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Maurean Guerreiro: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Margaux Mathieu-Resuge: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Fabienne Le Grand: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Victor Simon: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Sophie Collet: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
José-Luis Zambonino-Infante: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Nelly Le Goïc: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Christophe Lambert: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Fleuriane Fernandes: AlgaeWales Research Group, Centre for Sustainable Aquatic Research (CSAR), Bioscience Department, College of Science, Swansea University, Singleton Park, Swansea SA2 8PP, UK
Alla Silkina: AlgaeWales Research Group, Centre for Sustainable Aquatic Research (CSAR), Bioscience Department, College of Science, Swansea University, Singleton Park, Swansea SA2 8PP, UK
Marcella Fernandes de Souza: Department of Green Chemistry & Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
Denis de la Broise: Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France

Sustainability, 2022, vol. 14, issue 21, 1-23

Abstract: The use of microalgae as a sustainable source of n-3 long-chain polyunsaturated fatty acids (LC-PUFA) as an alternative to fish oils from small pelagic fish (e.g., anchovy, sardine) has received growing interest in the past few years. The present study aimed to: (i) produce Aurantiochytrium mangrovei biomass by heterotrophic fermentation using a medium containing anaerobic digestion liquid effluent, and (ii) evaluate a biomass rich in n-3 LC-PUFA and good quality proteins as a feed ingredient for sea bass juveniles and larvae. Two 800 L bioreactors were used to produce Aurantiochytrium biomass in non-axenic conditions. Biomass was then filtered through a crossflow filtration system (300 Kda ceramic membrane) and freeze-dried. Sea bass juveniles (32.7 ± 4.2 g) were fed both a control diet and a diet containing 15% of freeze-dried A. mangrovei biomass for 38 days. Juvenile survival percentage was 90% on average in both dietary conditions. Similar growth was observed between fish fed with both diets, demonstrating the feasibility to replace 15% of a standard fish feed by Aurantiochytrium biomass. The liver of sea bass juveniles fed with the A. mangrovei diet contained significantly higher proportions of 22:6n-3, 22:5n-6, and 20:4n-6 than those fed with the control diet, while the proportions of 16:0, 16:1n-7, and 18:1n-9 were significantly lower. The secondary oxidation, as measured by malonylaldehyde (MDA) content, in the liver and muscle of juveniles fed with the microalgae diet tended to be higher than in fish fed the control diet, but the differences were not statistically significant. Although the larvae survival percentage was low for all the tanks after 41 days of rearing, the inclusion of 15% of hydrolyzed A. mangrovei biomass in the larvae micro-diet did not impair the development of sea bass larvae and only marginally affected their lipid composition. In the future, we have to further optimize a sustainable workflow between Aurantiochytrium cultivation and fish feed production and confirm the zootechnical and biochemical results.

Keywords: aquaculture; nutrition; sustainability; n-3 long-chain polyunsaturated fatty acids; Thraustochytrids; microalgae; fish oil (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
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