Enhancement of Wheat Growth by UV-Mutagenesis of Potential Chromium Tolerant Bacillus sp. Strain SR-2-1/1

Muhammad Shahid, Muhammad Usman, Tanvir Shahzad, Iftikhar Ali, Muhammad Umair Hassan, Faisal Mahmood () and Sameer H. Qari
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Muhammad Shahid: Department of Bioinformatics & Biotechnology, Government College University Faisalabad, Faisalabad 38040, Pakistan
Muhammad Usman: Department of Bioinformatics & Biotechnology, Government College University Faisalabad, Faisalabad 38040, Pakistan
Tanvir Shahzad: Department of Environmental Sciences, Government College University Faisalabad, Faisalabad 38040, Pakistan
Iftikhar Ali: State Key Laboratory of Molecular Development Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences (CAS), Beijing 100101, China
Muhammad Umair Hassan: Research Center on Ecological Sciences, Jiangxi Agricultural University, Nanchang 330045, China
Faisal Mahmood: Department of Environmental Sciences, Government College University Faisalabad, Faisalabad 38040, Pakistan
Sameer H. Qari: Department of Biology, Al-Jumum University College, Umm Al-Qura University, Makkah 21955, Saudi Arabia

Sustainability, 2022, vol. 14, issue 22, 1-13

Abstract: UV mutagenesis has long been known to improve bacterial strains in their physiological capacity. In the current study, we used the UV mutagenesis approach to increase the PGPR characteristics of a pre-characterized metal-tolerant PGPR strain Bacillus sp. strain SR-2-1/1 (KY315919), with the objective of increasing the physiological outcome of its PGPR traits in vitro and post inoculation on wheat plants. After UV irradiation, the results implied a substantial in vitro increase in the phosphate solubilization and ammonia production of two selected mutant strains (M 1 and M 2 ) as compared to the wild-type strain SR-2-1/1; however, the ACC deaminase enzyme activity was completely lost in the mutant strains, which were originally present in the wild-type strains. However, the UV mutagenesis did not affect the taxonomy of these mutant strains. Moreover, the mutant strains M 1 and M 2 survived in the rhizosphere of wheat plants up to 30 days at an optimum (approximately 7–7.5 Log CFU/mL of rhizosphere soil) population density. The fresh and dry biomass, as well as root and shoot length, of wheat plants inoculated with one of the mutant strains M 2 were significantly higher than in the wheat plants inoculated with wild-type strain SR-2-1/1. The overall results imply that the resulted mutant M 2 was a physiologically competent PGPR strain, which could be tested in field experiments as an inoculum.

Keywords: mutagenesis; PGPR; Bacillus; rhizosphere; wheat growth (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
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