“Nature-like” Cryoimmobilization of Phototrophic Microorganisms: New Opportunities for Their Long-Term Storage and Sustainable Use

Olga Senko, Nikolay Stepanov, Olga Maslova and Elena Efremenko
Additional contact information
Olga Senko: Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia
Nikolay Stepanov: Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia
Olga Maslova: Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia
Elena Efremenko: Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia

Sustainability, 2022, vol. 14, issue 2, 1-16

Abstract: It was found that immobilization of cells in poly(vinyl alcohol) (PVA) cryogel can be successfully applied for concurrent cryoimmobilization, cryoconservation and long-term storage of the cells of various phototrophic microorganisms (green and red microalgae, diatoms and cyanobacteria). For the first time, it was shown for 12 different immobilized microalgal cells that they can be stored frozen for at least 18 months while retaining a high level of viability (90%), and can further be used as an inoculum upon defrosting for cell-free biomass accumulation. Application of cryoimmobilized Chlorella vulgaris cells as inocula allowed the loading of a high concentration of the microalgal cells into the media for free biomass accumulation, thus increasing the rate of the process. It was shown that as minimum of 5 cycles of reuse of the same immobilized cells as inocula for cell accumulation could be realized when various real wastewater samples were applied as media for simultaneous microalgae cultivation and water purification.

Keywords: phototrophic microorganisms; cryoimmobilization; poly(vinylalcohol) cryogel; cryoconservation; wastewater treatment; microalgal inoculum; long-term storage (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2071-1050/14/2/661/pdf (application/pdf)
https://www.mdpi.com/2071-1050/14/2/661/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jsusta:v:14:y:2022:i:2:p:661-:d:719879

Access Statistics for this article

Sustainability is currently edited by Ms. Alexandra Wu

More articles in Sustainability from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().