The Activity and Gene Expression of Enzymes in Mycelia of Pleurotus Eryngii under Cadmium Stress

Xiao-Hui Huang, Ning Xu, Li-Guo Feng, Deng-Ni Lai, Fang Wu, Dong Xu and Xin Guo
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Xiao-Hui Huang: Hunan Edible Fungi Institute, Changsha 410013, China
Ning Xu: Hunan Edible Fungi Institute, Changsha 410013, China
Li-Guo Feng: Hunan Edible Fungi Institute, Changsha 410013, China
Deng-Ni Lai: Hunan Agricultural Products Processing Institute, Hunan Food Test and Analysis Center, Changsha 410004, China
Fang Wu: Hunan Edible Fungi Institute, Changsha 410013, China
Dong Xu: National Engineering Laboratory for Deep Processing of Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry & Technology, Changsha 410004, China
Xin Guo: College of Sciences, Central South University of Forestry and Technology, Changsha 410004, China

Sustainability, 2022, vol. 14, issue 7, 1-13

Abstract: Cadmium (Cd) is a highly toxic pollutant and a nonessential element for plant growth. Here, we investigated the levels of malondialdehyde (MDA), H 2 O 2 , sugars, and the activity and gene expression of antioxidant enzymes and sugar metabolism-related enzymes in the mycelia of Pleurotus eryngii grown in the presence of 0, 0.5, 1, 1.5, 2.0, and 4.0 mg·L −1 Cd. Raising Cd concentration resulted in increases, followed by decreases in the levels of malondialdehyde (MDA) and H 2 O 2 in the mycelia. The maximum concentrations of MDA and H 2 O 2 reached 318.61 and 7411.40 μmol·L −1 , respectively, at a Cd concentration of 1 mg·L −1 . Antioxidative enzymes (superoxide dismutase (SOD), catalase, glutathione peroxidase (GPX), and ascorbate peroxidase (APX)) activities peaked at 1 mg·L −1 Cd. Furthermore, Pleurotus eryngii produced trehalose, glucose, fructose, and maltose in the fermentation broth in response to Cd stress. The levels of trehalose, arabinose, and fructose in the fermentation broth decreased continuously. The levels of glucose increased continuously, whereas those of maltose first increased and then decreased. Gene expression level of chitin synthase, glucose-1-phosphate uridyl transferase peaked significantly at 2 mg·L −1 of Cd, while that of trehalase 2 is 0.5 mg·L −1 , and those for fructose-diphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, reached the maximum at 4 mg·L −1 .

Keywords: Cd stress: Pleurotus eryngii; antioxidative enzymes; sugar metabolism; gene expression (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
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