Effects of Temperature, pH, and Salinity on Seed Germination of Acinos alpinus subsp. Meridionalis and FTIR Analysis of Molecular Composition Changes

Mustapha Cherrate, Nabil Radouane, Said Ezrari, Ghizlane Echchgadda, Abdellah Maissour, Kacem Makroum, Gabriel Plavan, Omar H. Abd-Elkader and Mohamed Bourioug ()
Additional contact information
Mustapha Cherrate: Laboratory of Plant Biotechnology, Ecology, and Ecosystem Valorization—URL-CNRST N°10, Faculty of Sciences, University Chouaïb Doukkali, El Jadida 24000, Morocco
Nabil Radouane: Laboratory of Functional Ecology and Environmental Engineering, Sidi Mohamed Ben Abdellah University, Route d’Imouzzer, P.O. Box 2202, Fez 30000, Morocco
Said Ezrari: Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Medicine and Pharmacy Oujda, University Mohammed Premier, Oujda 60000, Morocco
Ghizlane Echchgadda: Department of Plant Protection and Environment, Ecology Unit, National School of Agriculture of Meknes, BP S 40, Meknes 50001, Morocco
Abdellah Maissour: Laboratory of Plant Biotechnology, Ecology, and Ecosystem Valorization—URL-CNRST N°10, Faculty of Sciences, University Chouaïb Doukkali, El Jadida 24000, Morocco
Kacem Makroum: Laboratory of Plant Biotechnology, Ecology, and Ecosystem Valorization—URL-CNRST N°10, Faculty of Sciences, University Chouaïb Doukkali, El Jadida 24000, Morocco
Gabriel Plavan: Department of Biology, Faculty of Biology, “Alexandru Ioan Cuza” University, Bvd. Carol I, No. 20A, 700505 Iasi, Romania
Omar H. Abd-Elkader: Physics & Astronomy Department, Science College, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Mohamed Bourioug: Department of Agronomy, National School of Agriculture, km. 10, Route Haj Kaddour, B.P. S/40, Meknes 50001, Morocco

Sustainability, 2023, vol. 15, issue 6, 1-12

Abstract: This study aimed to determine the impact of three abiotic factors (Temperature, pH, and salinity) on the metabolic activities (macromolecules) and germination rate of Acinos alpinus subsp. Meridionalis ( Satureja ) seeds. In vitro, seed germination tests were performed in an aqueous medium. They were monitored as a function of time at different levels of temperature, NaCl concentration, and pH. The best germination rate (85.3%) was achieved at 15 °C and pH = 7. However, the germination was nil at a higher temperature (more than 25 °C), acidic pH (pH < 3.5), and higher NaCl concentration (more than >7.5 g L −1 ). Fourier transform infrared spectroscopy (FTIR) analysis showed an important variability of the chemical composition of germinated seeds. Indeed, the comparison of the absorbance peaks of chemical compounds in the treatments versus the control revealed significant differences in their concentrations and structures, which may justify why seeds fail to germinate under some extreme abiotic conditions. The results of this study are expected to serve as a guide for the protocols to be adopted in the ex situ conservation of this species.

Keywords: Acinos alpinus subsp. Meridionalis; Satureja; germination; abiotic factors; FTIR (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2023
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