Hydrolysis Optimization of Thraustochytrid (Heterotrophic Protist) Biomass for the Production of a New and Sustainable Ingredient for the Aquafeed Industry

Mariana Ventura (), Cloé Oroy, Natalia Llopis Monferrer, Fabienne Le Grand, Marc Long, Valentin Foulon, Luc Chauchat, Denis de la Broise, Fabienne Guerard and Philippe Soudant ()
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Mariana Ventura: Discovery & Front-End Innovation, Lesaffre Institute of Science & Technology, Lesaffre International, 59700 Marcq-en-Barœul, France
Cloé Oroy: Symrise Pet Food, Z.A. du Gohelis, 56250 Elven, France
Natalia Llopis Monferrer: Univ Brest, CNRS, IRD, Ifremer, LEMAR, 9280 Plouzané, France
Fabienne Le Grand: Univ Brest, CNRS, IRD, Ifremer, LEMAR, 9280 Plouzané, France
Marc Long: Univ Brest, CNRS, IRD, Ifremer, LEMAR, 9280 Plouzané, France
Valentin Foulon: ENIB, UMR CNRS 6285 LabSTICC, 29238 Brest, France
Luc Chauchat: Univ Brest, CNRS, IRD, Ifremer, LEMAR, 9280 Plouzané, France
Denis de la Broise: Univ Brest, CNRS, IRD, Ifremer, LEMAR, 9280 Plouzané, France
Fabienne Guerard: Univ Brest, CNRS, IRD, Ifremer, LEMAR, 9280 Plouzané, France
Philippe Soudant: Univ Brest, CNRS, IRD, Ifremer, LEMAR, 9280 Plouzané, France

Sustainability, 2024, vol. 16, issue 14, 1-17

Abstract: Thraustochytrids biomass, rich in docosahexaenoic acid (DHA), could be a sustainable ingredient for the aquafeed industry. However, its production cost renders it less competitive than fishmeal and fish oil from wild-caught fish. This study proposes optimizing the hydrolysis conditions of thraustochytrid biomass to generate an ingredient with improved properties thanks to the production of peptides with different biological activities. The improved nutritional value of the biomass could justify its use to decrease the amount of wild-caught fish in aquafeed, which would enhance the sustainability of fish aquaculture. First, two commercial proteases, Papain and Alcalase 2.4L, were compared for their capacity to hydrolyze the biomass. The best degree of hydrolysis (19 ± 1%) was obtained with Alcalase 2.4L, which was then used for the optimization of the pH, enzyme concentration and reaction time using response surface methodology. The results showed that the highest concentration of peptides and DHA in the aqueous phase was obtained with a pH, enzyme concentration and reaction time of 7.5, 2.7% and 205 min, respectively. If thraustochytrid hydrolysates prove to improve fish fitness, this mild and free-solvent process protocol could be used to produce a sustainable ingredient for aquafeed.

Keywords: thraustochytrids; peptides; enzymatic hydrolysis; DHA; Alcalase (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2024
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