Isolation and Identification of Multi-Traits PGPR for Sustainable Crop Productivity Under Salinity Stress

Md. Injamum-Ul-Hoque, Muhammad Imran, Nazree Zainurin, Shifa Shaffique, Sang-Mo Kang, S. M. Ahsan, Peter Odongkara and In-Jung Lee ()
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Md. Injamum-Ul-Hoque: Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea
Muhammad Imran: Biosafety Division, National Institute of Agricultural Sciences, 370, Jeonju 54874, Republic of Korea
Nazree Zainurin: Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea
Shifa Shaffique: Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea
Sang-Mo Kang: Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea
S. M. Ahsan: Department of Plant Medicals, Andong National University, Andong 36729, Republic of Korea
Peter Odongkara: Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea
In-Jung Lee: Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea

Sustainability, 2024, vol. 16, issue 21, 1-17

Abstract: High salinity poses a significant threat to arable land globally and contributes to desertification. Growth-promoting rhizobacteria assist plants in mitigating abiotic stresses and enhancing crop productivity through the production of siderophores, exopolysaccharides (EPS), solubilisation of phosphate, indole-3-acetic acid (IAA), and other secondary metabolites. This study aimed to isolate, identify, and characterise bacteria that exhibit robust growth-promoting properties. A total of 64 bacterial isolates from the rhizosphere of Miscanthus sinensis were evaluated for plant growth-promoting (PGP) traits, including IAA, EPS, siderophores, and solubilisation of phosphate. Among them, five isolates were selected as plant growth-promoting rhizobacteria (PGPR) based on their PGP features and identified via 16S rRNA sequencing: Enterococcus mundtii strain INJ1 (OR122486), Lysinibacillus fusiformis strain INJ2 (OR122488), Lysinibacillus sphaericus strain MIIA20 (OR122490), Pseudomonas qingdaonensis strain BD1 (OR122487), and Pseudomonas qingdaonensis strain MIA20 (OR122489), all documented in NCBI GenBank. BD1 demonstrated a higher production of superoxide dismutase (SOD) (17.93 U/mg mL), catalase (CAT) (91.17 U/mg mL), and glutathione (GSH) (0.18 U/mg mL), along with higher concentrations of IAA (31.69 µg/mL) and salicylic acid (SA) (14.08 ng/mL). These isolates also produced significant quantities of amino and organic acids. BD1 exhibited superior PGP traits compared to other isolates. Furthermore, the NaCl tolerance of these bacterial isolates was assessed by measuring their growth at concentrations ranging from 0 to 200 mM at 8-h intervals. Optical density (OD) measurements indicated that BD1 and INJ2 displayed significant tolerance to salt stress. The utilisation of these isolates, which enhances plant growth and PGP traits under salt stress, may improve plant development under saline conditions.

Keywords: PGPR; salt stress; phytohormones; antioxidants; amino acids; organic acids (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2024
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