A Study of the System Performance and the Microbial Community Composition of Chemical Wastewater in an AO-MBBR Treatment Process

Jiancheng Wang, Dongdong Yang, Qing Qing, Yue Zhang, Jie Zhu and Liqun Wang ()
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Jiancheng Wang: College of Biological and Food Engineering, Changzhou University, Changzhou 213164, China
Dongdong Yang: College of Biological and Food Engineering, Changzhou University, Changzhou 213164, China
Qing Qing: College of Biological and Food Engineering, Changzhou University, Changzhou 213164, China
Yue Zhang: College of Biological and Food Engineering, Changzhou University, Changzhou 213164, China
Jie Zhu: College of Biological and Food Engineering, Changzhou University, Changzhou 213164, China
Liqun Wang: College of Biological and Food Engineering, Changzhou University, Changzhou 213164, China

Sustainability, 2024, vol. 16, issue 9, 1-13

Abstract: To improve the nitrogen removal and reduce the chemical oxygen demand (COD) of a full-scale wastewater treatment plant, two sequential batch reactor devices were used to treat chemical wastewater with biocarriers in low carbon-to-nitrogen (C/N) ratio conditions. The results showed that the addition of biocarriers to the anoxic tank reduced the average concentration of COD in the effluent from 98.1 mg/L to 80.7 mg/L and increased total nitrogen (TN) removal by 9.4%. Metagenomic sequencing was performed to study the composition and function of microbial community samples taken from anoxic sludge and anoxic-carrier biofilms in this wastewater treatment plant. The results showed that Proteobacteria and Actinobacteria were the dominant phyla in the two samples, ensuring their capability for organic matter removal. The anoxic-carrier biofilms were mainly enriched with denitrifying bacteria such as Thauera (10.7%) and Comammonas (2.2%) and the anammox bacteria Candidatus Kuenenia (0.03%). Meanwhile, the nitrogen metabolism pathway was elaborated and the abundance of the functional genes involved in the nitrogen metabolism pathway was quantified. In addition, results from qPCR showed increased copy numbers of denitrification and anammox genes in the anoxic-carrier biofilms compared to those in the anoxic sludge, further confirming the enrichment of functional bacteria.

Keywords: biocarriers; metagenomic sequencing; microbial community; nitrogen metabolism pathway (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2024
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