In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

Francisca Sã¡, Jorge Porto, Alone Brito, José Santana, Rafaeli Souza and Francyane Braga

Journal of Agricultural Science, 2018, vol. 10, issue 4, 402

Abstract: This study aimed to develop efficient protocols for the in vitro micropropagation of Byrsonima gardneriana. Nodal segments were obtained from seedlings germinated in vitro with 60 days of life. These were inoculated in MS/2 supplemented with 87.64 µM of sucrose and solidified with 0.7% of agar, supplemented with different concentrations of cytokinin 6-benzylaminopurine (0.0; 2.0; 4.0 and 8.0 µM) associated with different concentrations of auxin, indole acetic acid (0.0; 0.5 and 1.0 µM) and naphthaleneacetic acid (0.0; 0.5 and 1.0 µM). The sprouting were individualized and transferred to MS/2 cultures with different concentrations of indole butyric acid (0.0; 1.0; 2.0 and 3.0 µM), and presence and absence of activated charcoal (1.0 g L-1). The use of concentrations from 2.0 to 4.0 µM 6-benzylaminopurine was efficient in the multiplication of B. gardneriana, given that, using concentrations above these, a decrease in this efficiency occurs. The use of auxin interfered negatively with the results. In vitro rooting occurs even in medium free of auxin. The activated charcoal was insufficient for rooting. The use of growth regulators 6-benzylaminopurine and indole butyric acid are efficient in micropropagation of B. gardneriana, however, further studies should be performed to optimize this protocol.

Date: 2018
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