Molecular Characterization of Isolates of Fusarium spp. Associated With Wilt in Capsicum spp

Isabela V. dos Anjos, Suelene S. de Melo, Thiago A. S. Gilio, Jessé P. Kreitlow, Sandra M. A. da S. Neves, Kelly L. Araújo, Milson E. Serafim and Leonarda G. Neves

Journal of Agricultural Science, 2024, vol. 11, issue 6, 519

Abstract: Fusarium is a diverse and heterogeneous fungi genus. Its wide genetic variability and similarity in morphological characteristics hinder the identification of species of this genus. Identifying Fusarium species is difficult due to the genus. Several molecular methods have been useful for differentiating these species, and the amplification of internal transcribed spacer (ITS) regions of the fungus ribosomal DNA has been successfully used, since ITS are preserved regions of the DNA that assists in distinguishing species. The objective of this work was to collect and characterize isolates of Fusarium spp. associated with wilt symptoms in Capsicum spp. in the biomes of the state of Mato Grosso, Brazil. Were collected tissue samples of plants with wilt symptoms. The DNAs of the Fusarium spp. found were extracted, and subjected to polymerase chain reaction, using the primers ITS1 (5’TCCGTAGGTGAACCTGCGG-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’). Subsequently, the sequencing was performed. The resulting sequences were, five Fusarium species were found F. solani, F. oxysporum, F. equiseti, F. incarnatum, F. chlamydosporum, predominating F. solani and F. equiseti.

Date: 2024
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://ccsenet.org/journal/index.php/jas/article/download/0/0/39292/40123 (application/pdf)
https://ccsenet.org/journal/index.php/jas/article/view/0/39292 (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:ibn:jasjnl:v:11:y:2024:i:6:p:519

Access Statistics for this article

More articles in Journal of Agricultural Science from Canadian Center of Science and Education Contact information at EDIRC.
Bibliographic data for series maintained by Canadian Center of Science and Education ().