Seed Viability and RNA Extraction Methods of Erythrina velutina Under Drought Stress

Juliana L. Souza, Bruno A. L. de Freitas, Laura C. D. P. Lima, Valdinete V. Nunes, Maria F. O. Torres and Renata Silva-Mann

Journal of Agricultural Science, 2024, vol. 12, issue 12, 192

Abstract: Abiotic stress caused by rigorous environmental conditions, such as drought, has negative effects on seeds. The species from arid and semiarid areas have mechanisms to maintain seed viability. In this study, the analyses of physical and physiological quality of the seeds of Erythrina velutina and their response to drought stress and RNA extraction methods were carried out. The seeds were collected from mother trees in two different provenances of natural occurrences of the species. The physical quality, viability, and vigor of the seeds were evaluated by morphometric parameters, germination, and electrical conductivity tests. The seeds were submitted to drought stress conditions for 0, 24, 48, and 72 hours on -0.8 MPa polyethylene glycol solution. To evaluate the viable RNA extraction method, the seed embryos were used for the extraction of this molecule and with an assessment of quantity and quality. The seeds from Pernambuco that were harvested in 2008 were bigger and presented lower vigor when in comparison to seeds that were harvested from Sergipe in 2012. The seeds under drought stress conditions presented null germination for all the treatments. After the drought stress, they were germinated on a paper substrate moistened with water, with better responses from the Lot Sergipe, 2012. The imbibition of seeds in the solution for 24 hours provides an increase in germination for less vigorous seeds, being indicated as a pre-germinative treatment. The RNA quality obtained by using a commercial extraction kit has better results, providing materials without contaminants when compared to the TRIzol method.

Date: 2024
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