Callogenesis and in vitro Regeneration of Baru (Dipteryx alata Vog.) Esprouts

Victória Maria Ingre Targa, Francilina Araújo Costa, Josimara Nolasco Rondon and Wagner Campos Otoni

Journal of Agricultural Science, 2024, vol. 13, issue 4, 128

Abstract: The aim of this study was to evaluate the effect of different concentrations of 6-benzylaminopurine (BAP) and naphthalene acetic acid (ANA) on calogenesis and regeneration from baru leaf and apex segments. The explants were obtained from baru plants previously established in vitro from almonds and cauline apices. The leaf segments were placed in Petri dishes containing MS medium (Murashige & Skoog, 1962) with concentrations (0.0, 2.0, 3.0, 4.0 and 5.0 mg L-1) BAP combined with ANA (0.0 and 2.0 mg L-1). The shoot apices were inoculated in test tubes with the same medium using the concentrations (0.0, 0.5, 1.0 and 1.5 mg L-1) BAP combined (0.0 and 0.1 mg L-1) ANA. After 25 days of inoculation, the percentages of callus and texture in leaf explants and apices were evaluated. The number of shoots was also evaluated by the Scott-Knott test at 5% of probability. The most efficient concentration in the formation of callus in leaves was 3.0 mg L-1 BAP + 2.0 mg L-1 ANA (68.88%), at apexes the most efficient concentration was 1.0 mg L-1 BAP without ANA with 100% calogenesis. The most effective concentration was 1.0 mg L-1 BAP without ANA with an average of 1.90 of shoots in relation to the concentrations evaluated.

Date: 2024
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