The Dynamic Nature of a Static Headspace Above Frozen Leaf Biomass From a Toxic Brazilian Plant

Mariah Ribeiro Rocha, Paulo de Moraes Ferreira, Márcio Wandré Morais de Oliveira, Maria Fátima Grossi de Sá and Thales Lima Rocha

Journal of Agricultural Science, 2024, vol. 15, issue 9, 105

Abstract: A static headspace method of sampling coupled to a conventional instrumental analytical probe, gas chromatography mass spectrometry (GCMS), has been applied to examine the volatile fraction above leaf tissue from Palicourea marcgravii, a toxic Brazilian plant that causes sudden death in livestock, and two other surrogate species (Lippia alba and Plectranthus spp.). The unusual total ion chromatogram obtained for the plant of interest is discussed and explained by considering the “dynamic” nature of the static headspace when intact cellular biomass is present and frozen leaves are analyzed concerning the presence and production of methyl salicylate (MeSA), which is this plants main toxin.

Date: 2024
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