Using PCR and RCA Techniques to Investigate the Variants of Cassava Mosaic Virus and Their Distribution in Ghana

Esther Afoley Annang, Allen Oppong, Ruth N. A. Prempeh, Esther Agyemang Marfo, Linda A. Abrokwah, Rachel Agyemang, Joseph N. L. Lamptey, Justin Pita and Lord J. J. Gowans

Journal of Agricultural Science, 2024, vol. 16, issue 7, 98

Abstract: Cassava mosaic disease (CMD) caused by cassava begomoviruses is the major constraint to cassava production in Ghana. The disease is known to cause reduction in root yield. To ascertain the distribution of viruses causing CMD, 95 diseased cassava samples were collected in two agroecological zones of Ghana-Deciduous Forest zone and the Transitional zone. On a scale of 1-5, CMD severity was scored. Mean CMD severity score was 2.9, however there was no significant difference (p > 0.05) between the zones. Averagely, CMD score of > 2.8 in 71% of farms visited was recorded. Polymerase chain reaction (PCR) and rolling circle amplification (RCA) were employed for virus identification. PCR revealed that African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV) and mixed infections were prevalent in both zones. EACMV-Cameroon strain was also identified to be common within these zones. The transitional zone had the highest percentage of CMD infection. Unamplified samples from PCR were amplified using rolling circle amplification (RCA) technique. Amplification and characterisation of complete genome sequences of two isolates were carried out. The complete genome of 2780 nucleotides from samples showed a high similarity to African cassava mosaic virus-Ghana (ACMV-GH). Sequences clustered with ACMV-Ivory Coast, ACMV Nigeria-Ogo, ACMV-BF, ACMV-UG having > 96% identity. This shows the close relation that exists amongst the ACMV strains in Africa. These findings highlight the need for a continuous survey of CMD to help manage the disease in the country.

Date: 2024
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://ccsenet.org/journal/index.php/jas/article/download/0/0/50317/54476 (application/pdf)
https://ccsenet.org/journal/index.php/jas/article/view/0/50317 (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:ibn:jasjnl:v:16:y:2024:i:7:p:98

Access Statistics for this article

More articles in Journal of Agricultural Science from Canadian Center of Science and Education Contact information at EDIRC.
Bibliographic data for series maintained by Canadian Center of Science and Education (jas@ccsenet.org).