Statistical Analysis of Main and Interaction Effects to Optimize Xylanase Production under Submerged Cultivation Conditions

Mullai P, N. Syed Fathima and Eldon Rene

Journal of Agricultural Science, 2010, vol. 2, issue 1, 144

Abstract: In recent years, xylanase has become an essential option for environmental friendly industrial biotechnologicalapplications and there is a rising demand for large scale production. In this study, a Bacillus species 2129 was tested forthe xylanase production under submerged cultivation conditions. Maximum xylanase activities were achieved using oatas the substrate and by optimizing process conditions such as substrate concentration, pH and nitrogen source usingstatistically significant design of experiments, employing the response surface methodology (RSM) concept. Underoptimized conditions there was an 8% increase in the enzyme activity and results from statistical approximation in theform of analysis of variance (ANOVA) shows that the squared effects of the variables were significant than both themain and interaction effects.

Date: 2010
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