Haplotype Analysis of Ferritin Heavy Chain Affects Stillborn Piglets on Large White X Landrace Crossbred Sows

Nakarin Pripwai and Supamit Mekchay

Journal of Agricultural Science, 2012, vol. 4, issue 1, 62

Abstract: The objective of this experiment was to identify the novel SNP on porcine FTH genes. Moreover, their association with litter size traits in commercial pigs will be analyzed. 1,361 Large White x Landrace crossbred sows were bled and DNA was extracted. The records included total number of piglets born (TNB), number of piglets born alive (NBA), number of piglets stillborn (SB) and number of piglets mummified (MM). The PCR primers were designed throughout the porcine FTH . PCR products were performed and screened for single nucleotide polymorphisms (SNPs) by using polymerase chain reaction – single strand conformation polymorphism (PCR-SSCP). Consequently, differentially heteroduplex shift were cloned and sequenced. SNPs including c.596C>T, c.634A>G c.677T>C and c.699T>C were found. According to these SNPs, there were proved by PCR-RFLP technique, BsuRI-c.596C>T, Hin6I-c.643A>G, MspI-c.677T>C and MspI-c.699T>C. Six different haplotypes for the SNPs of MspI at nucleotide position MspI–c.677T>C and MspI-c.699T>C were presented. The TCTC haplotype was significantly associated with increasing SB. It was conclude that MspI-FTH haplotype significantly associated with SB in commercial pigs which may used for marker-assisted selection on pig breeding program.

Date: 2012
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