Rapid Detection and Differentiation of Three Cucurbit-infecting Poleroviruses by Multiplex RT–PCR
Qiaoxia Shang,
Haiying Xiang,
Dawei Li,
Jialin Yu and
Chenggui Han
Journal of Agricultural Science, 2012, vol. 4, issue 4, 209
Abstract:
A multiplex reverse transcription polymerase chain reaction (mRT-PCR) method was developed and optimized for the simultaneous detection and differentiation of three poleroviruses infecting cucurbits. Cucurbit aphid-borne yellows virus (CABYV), Melon aphid-borne yellows virus (MABYV) and Suakwa aphid-borne yellows virus (SABYV) could be differentiated simultaneously using four optimized specific oligonucleotide primers, including one universal primer for detecting poleroviruses and three virus-specific primers. Amplification of three target viruses was also optimized by increasing the PCR annealing temperatures. The mRT-PCR products consisted of fragments of 700 base pairs (bp) for CABYV, 450 bp for MABYV and 950 bp for SABYV. Detection limits of the RNA quantity for mRT-PCR were 1 pg for CABYV, 0.1 pg for MABYV and 1 pg for SABYV. No specific products could be amplified from RNA of other non-target poleroviruses. The mRT-PCR was found to be a specific, sensitive and cost-effective method for detecting multiple poleroviruses in cucurbits.
Date: 2012
References: View complete reference list from CitEc
Citations:
Downloads: (external link)
https://ccsenet.org/journal/index.php/jas/article/download/12778/10190 (application/pdf)
https://ccsenet.org/journal/index.php/jas/article/view/12778 (text/html)
Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.
Export reference: BibTeX
RIS (EndNote, ProCite, RefMan)
HTML/Text
Persistent link: https://EconPapers.repec.org/RePEc:ibn:jasjnl:v:4:y:2012:i:4:p:209
Access Statistics for this article
More articles in Journal of Agricultural Science from Canadian Center of Science and Education Contact information at EDIRC.
Bibliographic data for series maintained by Canadian Center of Science and Education ().