PCR-RFLP Analysis of cpDNA in Tea Cultivars (Camellia sinensis L.) in Sichuan of China

Shengxiang Chen, Guinian Qi, Huan Li, Yao Zou and Hongli Shan

Journal of Agricultural Science, 2012, vol. 4, issue 5, 25

Abstract: The genetic diversity among 30 tea cultivars in Sichuan of China was investigated by PCR-RFLP analysis of cp DNA. 7 sets of chloroplast primers could produce one or more than one distinct bands by direct electrophoresis in 2% agrose gels. After the amplified products were digested by 10 restriction enzymes, a total of 135 bands were detected, among which 98 bands (72.59%) were polymorphic. The cpDNA PCR-RFLP based genetic distance (GD) among 30 tea accessions ranged from 0 to 0.071, with the mean of 0.049.

Date: 2012
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