Cloning, Expression and Characterization of PnLOX2 Gene Related to Aspergillus flavus-resistance from Peanut (Arachis hypogaea L.) Seed Coat

Caixia Yan, Chunjuan Li, Shubo Wan, Tingting Zhang, Yixiong Zheng and Shihua Shan

Journal of Agricultural Science, 2012, vol. 4, issue 7, 67

Abstract: Aspergillus flavus is one of the major diseases of peanut. The objective of the study was to clone PnLOX2 gene from peanut seed coat and analyze its expression. Real-time RT-PCR analysis indicated that the gene was overexpressed in the challenge of Aspergillus flavus and the greatest expression occurred 10 days after inoculation. The full length of PnLOX2 gene was 2592bp and encoded a 97.5kDa protein containing 863 amino acids. The fusion protein (apparent 121.5kDa) only existed in the precipitation and the maximum was obtained by inducing at 37ºC for 3.5h. As a result of response to the challenge of Aspergillus flavus, PnLOX2 gene was more greatly expressed in the resistant genotype than in the susceptible genotype. It indicated that PnLOX2 gene was closely related to Aspergillus flavus-resistance of peanut. Further investigations were needed to bacteriostatically identify the gene in vitro.

Date: 2012
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://ccsenet.org/journal/index.php/jas/article/download/15481/11489 (application/pdf)
https://ccsenet.org/journal/index.php/jas/article/view/15481 (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:ibn:jasjnl:v:4:y:2012:i:7:p:67

Access Statistics for this article

More articles in Journal of Agricultural Science from Canadian Center of Science and Education Contact information at EDIRC.
Bibliographic data for series maintained by Canadian Center of Science and Education ().