Optimization of In-Vitro Propagation of Cassava (Manihot esculenta Crantz) Genotypes

Mapayi E. F., Ojo D. K., Oduwaye O. A. and Porbeni J. B. O.

Journal of Agricultural Science, 2013, vol. 5, issue 3, 261

Abstract: Three cassava genotypes (92/0326, 95/0289 and Tropical Manioc Selection-International Institute of Tropical Agriculture-30572 (I-30572) were examined in sixteen (16) different culture media with different concentration of growth hormones, Naphaleneacetic acid (NAA-auxins) and 6 benzyl-aminopurine (BAP-cytokinins). The responses of the explants were evaluated by nodal length, survival, leaf and root formation, plant height, root and leaf number. Additive Main Effect and Multiplicative Interaction (AMMI) analysis was used to reveal the pattern of interaction of genotype by media-environment. The analysis of variance showed significant (P < 0.05) genotype performance with significant genotype growth differences among the different media. Highest percentage survival was recorded for 95/0289 (98%). Medium composition 1 (Murashige and Skoog (MS)- 4.43 g/l; NAA- 0.01 mg/l; BAP- 0.05 mg/l) and 4 (MS- 2.22 g/l; NAA- 0.02 mg/l; BAP- 0.10 mg/l) were observed to give 100% plantlets survival. Genotype 92/0326 had the highest mean plant height (1.49 cm) and mean root number (0.82). As the best performer, full strength MS is recommended in a semi-solid medium. However, the cost-benefit and availability has to be considered. These observations, attributed to the different genetic structure and contrasting levels of hormones used, can be useful for formulating protocols that are adequately potent for microprpagation of recalcitrant cassava genotypes.

Date: 2013
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