Effect of 6-BA on the Plant Regeneration via Organogenesis from Cotyledonary Node of Cowpea (Vigna unguiculata L. Walp)

Manman Tie, Qian Luo, Yanxia Zhu and Huanxiu Li

Journal of Agricultural Science, 2013, vol. 5, issue 5, 1

Abstract: The present study compares effects of different concentrations of 6-BA on regeneration from cotyledonary node explants of cowpea (Vigna unguiculata L. Walp). The seeds were inoculated on MSB5 medium [Murashige and Skoog (1962) salts and Gamborg B5 vitamins (1968)] containing different concentrations (0, 1, 2, 3, 4, 5 mg/L) of 6-BA for 4 days. The cotyledonary node explants with one cotyledon excised from 4-day-old seedlings, placed in vitro on MSB5 medium containing 6-BA at different dose (0, 0.5, 1.0, 1.5 mg/L) for shoot induction and elongation. Best response in terms of shoot number and shoot length were obtained with explants derived from seedling preconditioning with 3mg/L 6-BA followed by the induction and elongation stage pretreated with 0.5mg/L 6-BA. The elongated shoots were rooted on MSB5 medium without hormone.

Date: 2013
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