Isolation of Endophytic Bacteria and Amplification of 16SrDNA on Citrus in Zhaoqing Districts

Chongbi Li, S. N. Liang, F. Y. Gao, K. N. Chen, J. Wang, M. Zhang, T. Sun and X. K. Mu

Journal of Agricultural Science, 2015, vol. 7, issue 12, 198

Abstract: Zhaoqing is the main source of Citrus in China. However, the reason of citrus Huanglongbing (HLB) is unclear and may be from endophyte. Therefore, the study of citrus endophyte is necessary. The experiment would be carried out by endophyte being isolated from citrus plant and amplifying the specific 16SrDNA fragment from endogenous bacteria to know the relation of citrus HLB to 16S rDNA of endogenous bacteria. The results showed that total 55 strains of endophytic bacteria were identified. And one specific fragment long of 1261 nt was got. Sequencing analysis showed for 91.3% homology to uncultured bacterium clone. And the 16SrDNA fragment was identified by PCR. The experiments would provide a theoretical basis for understanding the differences in pathomechanism among various HLB isolates.

Date: 2015
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://ccsenet.org/journal/index.php/jas/article/download/53108/29210 (application/pdf)
https://ccsenet.org/journal/index.php/jas/article/view/53108 (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:ibn:jasjnl:v:7:y:2015:i:12:p:198

Access Statistics for this article

More articles in Journal of Agricultural Science from Canadian Center of Science and Education Contact information at EDIRC.
Bibliographic data for series maintained by Canadian Center of Science and Education ().