Modification of American Institute of Nutrition Rat Diet in Mammary Cancer Research

Hiba Al-Sayyed and Hamed Takruri

Journal of Agricultural Science, 2016, vol. 8, issue 5, 173

Abstract: This research was performed to test the effect of date palm fruit (Phoenix datylifera L.) of (Al-Barhi) type at the two maturity stages “Rutab†and “Tamr†on the 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary cancer using the rat model. The experimental diets included soybean group as a control group since soybean is known to be protective against mammary cancer.The diets, except for the soybean diet, were a modification of the American Institute of Nutrition-1993 (AIN-93) diet that is free of any soybean products. Soybean oil was replaced by a mixture of canola and sunflower oils in amounts that give ratios of w-6-w-3 fatty acids and PUFA-SFA similar to those of the AIN-93 diet which are based on soybean oil namely 7.5-1.0 and 4.0-1.0 respectively.It is concluded that in DMBA-induced mammary cancer experiments, a mixture of canola and sunflower oils in a ratio of 0.6-1.0 (v/v) canola oil-sunflower oil would replace soybean oil.

Date: 2016
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