Simultaneous Determination of Four Tetracycline Antibiotics Residues in Chicken by Solid Phase Extraction Coupled with High Performance Liquid Chromatography

Ming Zhao, Yiting Li, Zhiqiang Ji, Xiuping Dou, Chaoling Zhang, Xiaohan Li, Shufang Liu, Li Zhao and Na Liu

Journal of Food Research, 2022, vol. 11, issue 4, 23

Abstract: Objective- To establish a simple and rapid method for simultaneous determination of Tetracycline, Oxytetracycline, Chlortetracycline and Doxycycline in chicken. Methods- After optimized sample pretreatment and chromatographic conditions, tetracycline antibiotics in spiked chicken were extracted by ultrasonic extraction with Na2EDTA-Mcllvaine buffer solution (pH=4.0), purified and enriched by HLB solid phase extraction column, separated by Shim-pack GIST C18 column (4.6×150 mm, 3 μm), methanol-acetonitrile-oxalic acid (1-2-7, V- V- V) as the mobile phase, the flow rate was 0.9 mL/min, the UV detection wavelength was 350 nm, and the peak area was quantified by external standard method. Results- The linearity of the four tetracycline antibiotics was good in the range of 0.05-1 mg/kg (r＞0.997), and the detection limits were 2.53 μg/kg ~ 5.94 μg/kg according to the S/N ratio (S/N) of 3. The relative standard deviations (RSD, n=6) of precision were 1.51%-4.80%. The recoveries were from 76.81% to 96.34%. Conclusion- The method has good selectivity, high accuracy and reproducibility, and is suitable for simultaneous determination of four tetracycline antibiotics in chicken.

Date: 2022
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