Adsorption of Biocellulose Nanofiber Tissue Engineering from Acetobacter Xylinum (Acetobacteraceae) Embedded Eggshell Membrane via Fermentation Process

Nussara Soontorntepwaraku, Neeranuch Wangrungroj and Nuchnapa Tangboriboon

Modern Applied Science, 2019, vol. 13, issue 10, 11

Abstract: Interest in the development of bio-based waste has increased exponentially in biomedical, biodental and bioengineering applications because such waste is nontoxic, biocompatible, easily formed, and has good physicochemical-mechanical-thermal properties, elasticity, high purity, no side effects and biodegradation. Eggshell membrane is a biopolymer network of protein fibers retaining albumin, collagen and amino acid. Eggshell membrane is a good food source for bacterial Acetobacter xylinum growth to make a strong biocellulose nanofiber composite for use in facial masks and tissue engineering. The current study added different amounts of eggshell membrane powder of 0% (Bio-0), 0.01% (Bio-01), 0.05% (Bio-05), 0.075% (Bio-075) and 0.10%wt (Bio-1) into biocellulose made from Acetobacter xylinum in coconut water medium via a fermentation process. The obtained facial mask encoded Bio-1 had good physicochemical and mechanical properties. The best sample has tensile strength, elongation at break and maximum force, and good adsorption encoded Bio-1 equal to 3.229 ± 0.297 MPa, 43.350 ± 8.400% 13.466±1.238 N, respectively.

Date: 2019
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