The Construction of pDH25-pcpC-Vgb as a Recombinant DNA System for the Intracellular Expression of Vitreoscilla Hemoglobin in Cephalosporium Acremonium

Yubin Liu, Qiang Li and Qipeng Yuan

Modern Applied Science, 2009, vol. 3, issue 5, 75

Abstract: A recombinant DNA system for the intracellular expression of a bacterial heme-binding protein (Vitreoscilla hemoglobin, Vgb) was constructed and named as pDH25-pcpC-Vgb. It could be introduced into a cephalosporin C-producing strain of Cephalosporium acremonium. The Vgb-expressing transformants will provide higher internal oxygen concentrations, which will cause higher yields of cephalosporin C.

Date: 2009
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