Isolation and Phylogenetic Analysis of Chromium(VI) Reducing Bacteria of a Magnetite Mine Drainage from Hebei China

Zhitang Lu, Xiufang An and Weiwei Zhang

Modern Applied Science, 2011, vol. 5, issue 2, 113

Abstract: A magnetite mine drainage sample collected from Han-Xing Iron mine area in Hebei Province was domesticated with potassium dichromate. Four kinds of media supplemented with 300mg/L chromium(VI) were used to isolate chromium(VI) tolerant bacteria. 10 Cr(VI) reducing strains which can tolerant at least 350 mg/L Cr(VI) and reduce the Cr(VI) efficently are screened by observing the growth station and measuring the remaining chromium (VI) concentration in media. 16S rDNA sequencing and phylogenetic analysis showed that Brevundimonas spp. are the main group of chromium(VI) tolerant bacteria in the magnetite mine drainage water sample.

Date: 2011
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